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Correlation of p53, MDM2 and p14ARF protein expression in human esophageal squamous cell carcinoma

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Abstract

Purpose

To determine the interrelationships of p53, MDM2, and p14ARF protein expression in primary esophageal squamous cell carcinoma (ESCC) and their prognostic value in ESCC.

Methods

In total, 119 patients treated for ESCC with esophagectomy were enrolled in this study. Demographic and clinical data including gender, age, depth of tumor invasion, lymph node involvement, and 5-year survival rate were collected by chart review. p53, MDM2, and p14ARF were detected immunohistochemically in the resected tumors to evaluate their usefulness as biomarkers of clinical outcome.

Results

p53, MDM2, and p14ARF were expressed in 61 (51.3%), 34 (28.6%), and 22 (18.5%) of 119 tumor specimens, respectively. Overall, p53 protein expression was positively correlated with MDM2 (P = 0.024) and p14ARF expression (P = 0.026). In addition, p14ARF expression was most often found in specimens that were positive for both p53 and MDM2. Changes in the p53, MDM2, and p14ARF protein levels were not correlated with 5-year survival rate.

Conclusions

Expression of p53 protein correlates with increased MDM2 and p14ARF protein levels in ESCC. In addition, status of p53 (wild-type versus mutant) rather than expression level of p53, MDM2, or p14ARF is likely to be the more critical determinant of clinical outcome.

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Acknowledgments

The study was supported by grants from the National Science Council of Taiwan to HS Hsu (NSC-96-2314-B-075-027) and TH Cheng (NSC-93-2320-B-010-063).

Conflict of interest statement

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Authors and Affiliations

  1. Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei, Taiwan, ROC

    Tzu-Hao Cheng

  2. Division of Thoracic Surgery, Department of Surgery, Taipei Veterans General Hospital, Taipei, Taiwan, ROC

    Po-Kuei Hsu, I-Chun Hung, Min-Hsiung Huang & Han-Shui Hsu

  3. Department of Pathology, Taipei Veterans General Hospital, Taipei, Taiwan, ROC

    Anna Fen-Yau Li

  4. Institute of Emergency and Critical Care Medicine, National Yang-Ming University School of Medicine, Taipei, Taiwan, ROC

    Han-Shui Hsu

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  1. Tzu-Hao Cheng
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  2. Po-Kuei Hsu
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  4. I-Chun Hung
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  5. Min-Hsiung Huang
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Correspondence to Han-Shui Hsu.

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Cheng, TH., Hsu, PK., Li, A.FY. et al. Correlation of p53, MDM2 and p14ARF protein expression in human esophageal squamous cell carcinoma. J Cancer Res Clin Oncol 135, 1577–1582 (2009). https://doi.org/10.1007/s00432-009-0605-5

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  • DOI: https://doi.org/10.1007/s00432-009-0605-5

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