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Integrin β3 down-regulates invasive features of ovarian cancer cells in SKOV3 cell subclones

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Abstract

Purpose

To investigate the role of integrin β3 in invasive features of ovarian cancer SKOV3 cells, by comparing different metastatic subclones.

Methods

In the present study, two cell subclones, termed as S1 and S21, which possessed high and low metastatic potential, respectively, were isolated and established from human ovarian cancer parental cell line SKOV3 by the limited dilution method. The expressions of integrin αv, integrin αvβ3, integrin β3, E-cadherin, FAK and ILK in the two cell subclones were compared by means of real-time RT-PCR or flow cytometry. Subsequently, S21 was transfected with siRNA for integrin β3 and the effects of transfection were examined by methyl thiazolyl tetrazolium (MTT) assay, colony formation assay, Matrigel invasion assay and cell migration assay.

Results

The expressions of integrin αvβ3, integrin β3 and E-cadherin were markedly down-regulated in S1; however, there were no significant differences in the expressions of integrin αv, FAK and ILK β. Of note, more than 70% knockdown of integrin β3 expression was obtained by siRNA technique. The integrin β3-siRNA-transfected cells showed significant increases in cell proliferation, cell migration and invasive activity in contrast with the mock-transfected cells. The expressions of integrin αvβ3 and E-cadherin were lower in the integrin β3-siRNA-transfected cells compared to the mock control.

Conclusion

Integrin β3, like E-cadherin, may be also a suppressor gene down-regulating invasive features of ovarian cancer cells in SKOV3 cell subclones.

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Acknowledgments

This study was supported by a grant-in-aid for scientific research (2007BS03025, Funds for Doctors) from the Department of Science and Technology of Shandong Province.

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Authors and Affiliations

  1. Department of Maternal and Child Health Care, School of Public Health, Shandong University, 250012, Jinan, China

    Jie Chen, Jun Li & Maosun Fu

  2. Central Laboratory, Shandong Provincial Hospital, Shandong University, 250021, Jinan, China

    Jie Zhang & Yaoran Zhao

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  1. Jie Chen
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Correspondence to Jie Chen.

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Chen, J., Zhang, J., Zhao, Y. et al. Integrin β3 down-regulates invasive features of ovarian cancer cells in SKOV3 cell subclones. J Cancer Res Clin Oncol 135, 909–917 (2009). https://doi.org/10.1007/s00432-008-0526-8

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  • DOI: https://doi.org/10.1007/s00432-008-0526-8

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