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HPV-16 E6 L83V variant in squamous cell carcinomas of the upper aerodigestive tract

  • Original Paper
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Abstract

Purpose

The aim of this prospective case series study was to determine the prevalence of HPV-DNA, analyze the E6 mRNA expression, identify intra-type variation in the E6 oncogene in upper aerodigestive tract (UADT) squamous cell carcinoma (SCC), and correlate the presence of HPV-DNA with several clinical parameters and outcome.

Methods

Frozen samples of UADT-SCC were analyzed for the presence and characterization of HPV-DNA and RNA sequences by means of polymerase chain reaction (PCR), reverse transcriptase-PCR, and direct sequencing of amplified products.

Results

HPV-DNA sequences were detected in 10% of the tumors, all of which were typed as HPV-16. Positivity for HPV-16 E6/E7 mRNA was observed in five of the eight HPV-positive tumors (62.5%). The HPV-16 E6 L83V variant was present in five cases. Multivariate analysis identified a history of absence of smoking (P = 0.009) as a predictor of HPV-positive tumor. No significant differences in overall and disease free survival curves were observed between patients with HPV-positive tumors and patients with tumors without detectable HPV-DNA.

Conclusion

Our findings support the etiological participation of HPV-16 in a subset of UADT-SCCs from patients lacking traditional risk factors. The potential prognostic significance of HPV-16 E6 L83V variant in HPV-16 positive UADT-SCCs should be more extensively investigated.

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Authors and Affiliations

  1. Dipartimento di Specialità Medico-Chirurgiche, Clinica Otorinolaringoiatrica-Centro Regionale per l’Oncologia Cervico-Facciale, Università degli Studi di Padova, Treviso, Italy

    Paolo Boscolo-Rizzo, Maria Cristina Da Mosto & Roberto Fuson

  2. Servizio Immunologia Diagnostica Molecolare Oncologica, Istituto Oncologico Veneto, IRCCS, Padua, Italy

    Helena Frayle-Salamanca, Rossana Trevisan & Annarosa Del Mistro

  3. Viale Umbria 6, Chioggia, Venice, 30019, Italy

    Paolo Boscolo-Rizzo

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  1. Paolo Boscolo-Rizzo
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  2. Maria Cristina Da Mosto
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  3. Roberto Fuson
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  4. Helena Frayle-Salamanca
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  5. Rossana Trevisan
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  6. Annarosa Del Mistro
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Correspondence to Paolo Boscolo-Rizzo.

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Boscolo-Rizzo, P., Da Mosto, M.C., Fuson, R. et al. HPV-16 E6 L83V variant in squamous cell carcinomas of the upper aerodigestive tract. J Cancer Res Clin Oncol 135, 559–566 (2009). https://doi.org/10.1007/s00432-008-0490-3

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  • DOI: https://doi.org/10.1007/s00432-008-0490-3

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