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Alcohol, smoking and human papillomavirus in laryngeal carcinoma: a Nordic prospective multicenter study

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Abstract

Purpose

Human papillomavirus (HPV) has been linked to oropharyngeal carcinomas, but its role in laryngeal squamous cell carcinoma (LSCC) is not clear. A prospective multicenter study based on known tumor-cell percentage of fresh frozen carcinoma biopsies was established to determine the HPV prevalence. Moreover risk factors such as smoking, alcohol abuse, chronic laryngitis and gastroesophageal reflux disease (GERD) were evaluated

Methods

Fresh-frozen laryngeal cancer biopsies from 108 patients in Finland, Norway, and Sweden were investigated. Patients whose biopsy samples contained at least 20% tumor tissue (N = 69) entered the study. HPV DNA was determined with MY09/11 and GP5+/6+ nested PCR and SPF10 PCR hybridization assay. Patients were examined by an ENT specialist and an extensive questionnaire concerning risk factors was filled in.

Results

Only three patients (4.4%) harbored HPV DNA in their carcinoma sample. Heavy alcohol drinking was associated with an increased risk of death, advanced-stage disease, and younger age at diagnosis. Chronic laryngitis, GERD, and orogenital sex contacts were rare. Poor oral hygiene was not associated with survival, although it correlated with heavy drinking.

Conclusion

In our series HPV was not important in LSCC. Heavy drinking led to major mortality in LSCC and promoted early carcinogenesis.

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Acknowledgments

We thank Nina Antikainen, Eija Nenye, and Irina Suomalainen for technical assistance.

Author information

Authors and Affiliations

  1. Department of Virology, Haartman Institute, University of Helsinki, Haartmaninkatu 3, P.O. Box 21, 00014, Helsinki, Finland

    Walter J. Koskinen & Antti Vaheri

  2. Department of Otorhinolaryngology—Head and Neck Surgery, Helsinki University Central Hospital, Helsinki, Finland

    Walter J. Koskinen, Antti A. Mäkitie & Leena-Maija Aaltonen

  3. Department of Otorhinolaryngology, Rikshospitalet, Oslo, Norway

    Kjell Brøndbo & Tov Røysland

  4. Department of Oncology, Karolinska University Hospital, Stockholm, Sweden

    Hanna Mellin Dahlstrand

  5. Finnish Cancer Registry, Helsinki, Finland

    Tapio Luostarinen & Timo Hakulinen

  6. Department of Pathology, University of Helsinki and Haartman Laboratory Diagnostics, Helsinki University Central Hospital, Helsinki, Finland

    Ilmo Leivo

  7. Delft Diagnostic Laboratory, Voorburg, The Netherlands

    Anco Molijn & Wim G. Quint

  8. Department of Otorhinolaryngology—Head and Neck Surgery, Karolinska University Hospital, Stockholm, Sweden

    Eva Munck-Wikland

  9. Department of Otorhinolaryngology, Tampere University Hospital, Tampere, Finland

    Ilmari Pyykkö

  10. Department of Medical Microbiology, Malmö University Hospital, Malmö, Sweden

    Joakim Dillner

Authors
  1. Walter J. Koskinen
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  2. Kjell Brøndbo
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  6. Ilmo Leivo
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  8. Wim G. Quint
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  9. Tov Røysland
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  10. Eva Munck-Wikland
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  11. Antti A. Mäkitie
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  12. Ilmari Pyykkö
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  13. Joakim Dillner
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  14. Antti Vaheri
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  15. Leena-Maija Aaltonen
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Corresponding author

Correspondence to Walter J. Koskinen.

Additional information

This work was supported by grants from Cancer Societies of Finland, Helsinki University Hospital Research Funds, Finnish Dental Society Apollonia, Stockholm Cancer Society and Laryngfonden, Sweden.

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Koskinen, W.J., Brøndbo, K., Mellin Dahlstrand, H. et al. Alcohol, smoking and human papillomavirus in laryngeal carcinoma: a Nordic prospective multicenter study. J Cancer Res Clin Oncol 133, 673–678 (2007). https://doi.org/10.1007/s00432-007-0219-8

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  • DOI: https://doi.org/10.1007/s00432-007-0219-8

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