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Serum CYFRA 21-1 level reflects hepatocellular carcinoma metastasis: study in nude mice model and clinical patients

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Abstract

Our previous proteomics study on human hepatocellular carcinoma (HCC) cell strains revealed that cytokeratin 19 (CK19) was expressed in cells with high metastasis potential; we further studied serum CK19 fragment CYFRA 21-1 level in HCC patients and nude mice model of HCC metastasis. HCC cell line HCCLM3 was injected subcutaneously into 30 nude mice which were then randomized into 6 groups of 5 mice each. The murine serum CYFRA 21-1 and pulmonary metastases were determined 2, 3, 4, 5, 6, and 7 weeks after injection. Serum CYFRA 21-1 levels of 101 normal controls and 108 HCC patients were also determined. In nude mice model, CYFRA 21-1 level increased significantly when pulmonary metastases occurred. Among 108 HCC patients, 24 (22.2%) had increased serum CYFRA 21-1 level. The presence of portal vein tumor emboli was significantly higher in CYFRA 21-1 increased cases (33.3%, 6/24) than in CYFRA 21-1 normal cases (6.0%, 5/84) (x 2 = 7.403, P < 0.01). In addition, the percentage of TNM stage III/IV tumor was significantly higher in CYFRA 21-1 increased patients (54.2%, 13/24) than in CYFRA 21-1 normal cases (21.4%, 18/84) (x 2 = 9.776, P < 0.005). These results suggest that CK19 may play an important role in HCC metastasis.

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Acknowledgments

This work was supported by New-Century Excellent Talents Supporting Program of the Ministry of Education (NCET-04-0669), and A Foundation for the Author of National Excellent Doctoral Dissertation of PR China (FANEDD) (200464).

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Authors and Affiliations

  1. Department of Oncology, Zhongnan Hospital of Wuhan University and Cancer Center of Wuhan University, No. 169 Donghu Road, Wuhan, 430071, China

    Yan Li

  2. Liver Cancer Institute and Zhongshan Hospital of Fudan University, No. 136 Yi Xue Yuan Road, Shanghai, 200032, China

    Yan Li, Zhao-You Tang, Bo Tian, Sheng-Long Ye, Lun-Xiu Qin, Qiong Xue & Rui-Xia Sun

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  1. Yan Li
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  2. Zhao-You Tang
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  3. Bo Tian
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Correspondence to Zhao-You Tang.

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Li, Y., Tang, ZY., Tian, B. et al. Serum CYFRA 21-1 level reflects hepatocellular carcinoma metastasis: study in nude mice model and clinical patients. J Cancer Res Clin Oncol 132, 515–520 (2006). https://doi.org/10.1007/s00432-006-0098-4

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  • DOI: https://doi.org/10.1007/s00432-006-0098-4

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