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Effect of inhibitors of arachidonic acid metabolism on prostaglandin E2 production by Candida albicans and Candida dubliniensis biofilms

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Abstract

Arachidonic acid (AA) is released from infected host cells during Candida albicans infection and may serve as carbon source for yeast growth and as precursor for the production of biologically active eicosanoids, such as prostaglandin E2 (PGE2) by C. albicans. However, the mechanism involved in this production is still unclear. Therefore, it was of interest to investigate the effect of different arachidonic acid metabolism inhibitors on PGE2 production by biofilms of C. albicans and the closely related C. dubliniensis. This was done by growing Candida biofilms in the presence of AA as well as cytochrome P450 (CYP), multicopper oxidase, cyclooxygenase or lipoxygenase inhibitors. The concentration of PGE2 was determined by a monoclonal PGE2 enzyme-linked immunosorbent assay and verified with LCMS/MS. The results obtained indicate the ability of C. albicans and C. dubliniensis biofilms to produce PGE2 from exogenous AA. The use of different inhibitors suggested that CYPs and multicopper oxidases are involved in PGE2 production by these Candida biofilms.

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Acknowledgments

The authors would like to thank the National Research Foundation, South Africa, for funding under the Thuthuka programme (Grant number TTK2007041000014) and the Blue Skies programme (Grant number BS2008092300002).

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Authors and Affiliations

  1. Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, PO Box 339, Bloemfontein, 9301, South Africa

    Ruan Ells, Johan L. F. Kock, Jacobus Albertyn, Gabré Kemp & Carolina H. Pohl

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  1. Ruan Ells
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  2. Johan L. F. Kock
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  3. Jacobus Albertyn
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  4. Gabré Kemp
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  5. Carolina H. Pohl
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Correspondence to Carolina H. Pohl.

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Ells, R., Kock, J.L.F., Albertyn, J. et al. Effect of inhibitors of arachidonic acid metabolism on prostaglandin E2 production by Candida albicans and Candida dubliniensis biofilms. Med Microbiol Immunol 200, 23–28 (2011). https://doi.org/10.1007/s00430-010-0169-7

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  • DOI: https://doi.org/10.1007/s00430-010-0169-7

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