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Diagnostic value of HCMV pp65 antigen detection by FCA for symptomatic and asymptomatic infection: compared to quantification of HCMV DNA and detection of IgM antibody in infants

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Abstract

Human cytomegalovirus (HCMV) can cause symptomatic or asymptomatic infection in infants. One hundred and twenty-six infants were assessed clinically for disease in infantile period. Eighty of them were classified as symptomatic infection on the basis of physical, instrumental, and laboratory findings, 5 were demonstrated by following up to have later developed HCMV disease, and the other 41 infants were classified as asymptomatic infection. HCMV DNA was positive in all urine samples of the symptomatic infants detected by quantitative polymerase chain reaction. HCMV-IgM antibody detected by chemiluminescent immunoassay (CLIA) was positive in 62 of the 85 symptomatic infants, but was negative in all of the samples of asymptomatic infants. HCMV pp65 antigen detected by flow cytometry assay (FCA) was positive in 77 of the 85 symptomatic infants and in none of the asymptomatic infants. The coincidence to symptom of HCMV pp65 antigen detection was higher than those of HCMV DNA and HCMV-IgM antibody detection. The sensitivity, specificity, positive prognostic value and the negative prognostic value of HCMV pp65 antigen detection for diagnosis of HCMV infection was 90.6, 100, 100 and 83.7%, respectively. We concluded that detection of pp65 antigen by FCA is more sensitive for diagnosis of HCMV infection than detection of HCMV-IgM antibody and is better than HCMV DNA quantification for distinguishing the symptomatic and asymptomatic HCMV infection in infants.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (30672248), National Natural Science Foundation of China (30770109) and the Project of Doctor Foundation of Liaoning Province in China (20061039).

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Authors and Affiliations

  1. Virus Laboratory, The Affiliated Shengjing Hospital, China Medical University, 110004, Shenyang, Liaoning, China

    Zhengrong Sun, Jidong Wang, Qing Liu, Yaohua Ji, Yanping Ma, Ying Qi, Rong He & Qiang Ruan

  2. Pediatric Department, The Affiliated Shengjing Hospital, China Medical University, 110004, Shenyang, Liaoning, China

    Xiangshi Ceng & Zhiqin Mao

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  1. Zhengrong Sun
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  2. Xiangshi Ceng
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  3. Zhiqin Mao
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  5. Qing Liu
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  7. Yanping Ma
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  9. Rong He
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  10. Qiang Ruan
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Correspondence to Qiang Ruan.

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Sun, Z., Ceng, X., Mao, Z. et al. Diagnostic value of HCMV pp65 antigen detection by FCA for symptomatic and asymptomatic infection: compared to quantification of HCMV DNA and detection of IgM antibody in infants. Med Microbiol Immunol 198, 107–112 (2009). https://doi.org/10.1007/s00430-009-0112-y

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  • DOI: https://doi.org/10.1007/s00430-009-0112-y

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