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Inhibition of hemagglutination activity of influenza A viruses by SP-A1 and SP-A2 variants expressed in CHO cells

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Abstract

Surfactant protein A (SP-A) inhibits hemagglutination (HA) activity and infectivity of influenza A viruses (IAV). As we have showed before in different assays, SP-A2 gene products are more active than SP-A1. Here, we hypothesized that SP-A1 and SP-A2 mammalian CHO-cell-expressed proteins also differentially modulate HA inhibition of IAV. We found that both SP-A1 and SP-A2 equally displayed α(2,3)-linked sialic acids, and had similar activity against a strain (PR-8) that preferentially binds to α(2,3)-linked sialic acids. Based on these findings, we speculate that in human lung SP-A1 and SP-A2 will not be different in their activity against IAV that preferably bind to α(2,3)-linked sialic acids (like avian strains).

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Acknowledgments

This work was supported by the National Institute of Environmental Health Sciences (RO1 ES009882-04A1) and HL69031 (KLH). The authors thank Dr Martin van Eijk for initial pilot experiments with glycan blots, and Karen Chorney for technical assistance.

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Authors and Affiliations

  1. Department of Cellular and Molecular Physiology, H166, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA , 17033, USA

    Anatoly N. Mikerov, Guirong Wang & Joanna Floros

  2. Department of Pediatrics, The Pennsylvania State University College of Medicine, Hershey, PA, 17033, USA

    Joanna Floros

  3. Department of Obstetrics and Gynecology, The Pennsylvania State University College of Medicine, Hershey, PA, 17033, USA

    Joanna Floros

  4. Department of Medicine, Section of Hematology/Oncology, Boston University School of Medicine, Boston, MA, USA

    Mitch White & Kevan Hartshorn

Authors
  1. Anatoly N. Mikerov
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  2. Mitch White
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  4. Guirong Wang
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  5. Joanna Floros
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Correspondence to Joanna Floros.

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Mikerov, A.N., White, M., Hartshorn, K. et al. Inhibition of hemagglutination activity of influenza A viruses by SP-A1 and SP-A2 variants expressed in CHO cells. Med Microbiol Immunol 197, 9–12 (2008). https://doi.org/10.1007/s00430-007-0051-4

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  • DOI: https://doi.org/10.1007/s00430-007-0051-4

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