Skip to main content

Advertisement

SpringerLink
Log in

Human papillomavirus 6/11, 16 and 18 in oral carcinomas and benign oral lesions

  • Original Investigation
  • Published:
Medical Microbiology and Immunology Aims and scope Submit manuscript

Abstract.

We have examined 118 oral squamous cell carcinomas, 72 oral leukoplakias, 12 cases of cheilitis and 65 of oral lichen planus for the presence of human papillomavirus (HPV) 6/11, 16 and 18 DNA by PCR/Southern blot hybridization. HPV DNA were found in 51/118 carcinomas (43.2%), in 16/72 (22.2%) leukoplakias, 3/12 (25.0%) cheilitic lesions and 10/65 (15.4%) lichen planus cases. These differences were even stronger when analyzing separately for the high-risk types HPV 16 and 18 as compared to low-risk types 6/11. HPV 16 and 18 DNA were present in 41/118 (34.7%) oral carcinomas, 12/72 (16.7%) leukoplakias, 2/12 (16.7%) cheilitic lesions and 6/65 (9.2%) lichen planus. In contrast to this, oral carcinomas displayed the lowest HPV 6/11 detection rate (4.2%), compared with 11.1% for leukoplakias, 8.3% for cheilitic lesions and 7.7% in lichen planus. These results indicate a successive increase of the detection rate of HPV 16 and 18 from low level in non or questionably preneoplastic lesions (lichen planus) to preneoplastic lesions (leukoplakia and cheilitis) and to oral carcinoma. In conclusion, our results suggest an association of oral carcinogenesis and infection with the high-risk HPV types 16 and 18.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.

Similar content being viewed by others

References

  1. Bofetta P, Mashberg A, Winkelman R, Garfinkel L (1992) Carcinogenic effect of tobacco smoking and alcohol drinking on anatomic sites of the oral cavity and oropharynx. Int J Cancer 52:530–533

  2. Cruz IB, Snijders PJ, Steenbergen RDM, Meijer CJLM, Snow GB, Walboomerss JMM, Waals I van der (1996 ) Age-dependence of human papillomavirus DNA presence in oral squamous cell carcinomas. Oral Oncol Eur J Cancer 32B:55–62

  3. Dao Ke L, Adler-Storthz K, Follen Mitchel M, Clayman GL, Chen Z (1999) Expression of human papillomavirus E7 mRNA in human oral and cervical neoplasia and cell lines. Oral Oncol 35:415–420

  4. De Villiers E-M (1989) Heterogeneity of human papillomavirus group. J Virol 63:4898–4903

  5. Elamin F, Steingrimsdottir H, Wanakulasuriya S, Johnson N, Tavassoli M (1998) Prevalence of human papillomavirus infection in premalignant lesions of the oral cavity in U.K. subjects: a novel method of detection. Oral Oncol 34:191–197

  6. Gassenmair A, Hornstein OP (1988) Presence of human papillomavirus DNA in benign and precancerous oral leukoplakias and squamous cell carcinomas. Dermatologica 176:224–233

  7. Hirayama T (1966) An epidemiological study of oral and pharyngeal cancer in Central and South-East Asia. Bull World Health Organ 34:41–69

  8. Lorincz AT, Schiffman MH, Jaffurs WJ, Marlow J, Quinn AP, Temple GF (1990) Temporal associations of human papillomavirus infection with cervical cytologic abnormalities. Am J Obstet Gynecol 162:645–651

  9. Miller CS, Johnstone BM (2001) Human papillomavirus as a risk factor for oral squamous cell carcinomas: a meta-analysis 1982–1997. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 91:622–635

  10. Nielsen H, Norrild B, Vedtofte P, Reibel J, Holmstrup P (1996) Human papillomavirus in oral premalignant lesions. Oral Oncol Eur J 32B:264–270

  11. Ostwald C, Müller P, Barten M, Rutsatz K, Sonnenburg M, Milde-Langosch K, Löning T (1994) Human papillomavirus DNA in oral squamous cell carcinomas and normal mucosa. J Oral Pathol Med 23:220–225

  12. Riethdorf S, Friedrich RE, Ostwald C, Barten M, Gogacz P, Gundlach KK, Schlechte H, Riethdorf L, Löning T (1997) p53 mutations and HPV infection in primary head and neck squamous cell carcinomas do not correlate with overall survival: a long-term follow-up study. J Oral Pathol Med 26:315–321

  13. Sand l, Jalouli J, Larsson P-A, Hirsch J-M (2000) Human papilloma viruses in oral lesions. 20:1183–1188

  14. Scheffner M, Munger K (1991) The state of p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc Natl Acad Sci USA 88:5523–5527

  15. Snijders PJF, Scholes AGM, Hart A, Jones AS, Vaughan D, Woolgar JA, Meijer CJLM, Walboomers JMM, Field JK (1996) Prevalence of mucoso-tropic human papillomaviruses in squamous cell carcinomas of the head and neck. Int J Cancer 66:464–469

  16. Steenbergen RDM, Hermsen MAJA, Walboomers JMM, Joenje H, Arwert F, Meijer CJLM, Snijders PJF (1995) Integrated human papillomavirus type 16 and loss of heterozygosity at 11q22 and 18q21 in an oral carcinoma and its derivative cell line. Cancer Res 55:5465–5471

  17. Suarez P, Batsakis JG, El-Naggar AK (1998) Leukoplakia: still a gallimaufry or is progress being made. Adv Anat Pathol 5:137–155

  18. Watts SL, Brewer E, Fry T (1991) Human papillomavirus DNA types in squamous cell carcinomas of the head and neck. Oral Surg Oral Med Oral Pathol 71:701–707

  19. Wen S, Tsuji T, Li X, Mizugaki Y, Hayatsu Y, Shinizaki F (1997) Detection and analysis of papillomavirus 16 and 18 homologous DNA sequences in oral lesion. Anticancer Res 17:307–312

  20. Werness BA, Levine AJ, Howley PM (1990) Association of human papillomavirus types 16 and 18 E6 proteins with p53. Science 248:76–79

  21. Woods KV, Shillitoe EJ, Spitz MR, Schwantz SP, Adler-Storthz K (1993) Analysis of human papillomavirus DNA in oral squamous cell carcinomas. J Oral Pathol Med 22:101–108

  22. Zur Hausen H (1994) Molecular pathogenesis of cancer of the cervix and its causation by specific HPV types. Curr Top Microbiol Immunol 186:131–156

Download references

Acknowledgement.

We thank S. Stegemann for excellent technical assistance.

Author information

Authors and Affiliations

  1. Institut für Pathologie, Universität Rostock, Strempelstrasse 14, 18055 Rostock, Germany

    Christiane Ostwald, Wolfgang Schmidt & Malte Barten

  2. Klinik und Poliklinik für Mund, Kiefer und Plastische Chirurgie, Universität Rostock, Strempelstrasse 13, 18055 Rostock, Germany

    Karin Rutsatz, Jens Schweder & Karsten Gundlach

Authors
  1. Christiane Ostwald
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Karin Rutsatz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jens Schweder
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wolfgang Schmidt
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Karsten Gundlach
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Malte Barten
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Malte Barten.

Additional information

Presented in part at the Joint Meeting of the 'Arbeitsgemeinschaft Dermatologische Infektiologie der Deutschen Dermatologischen Gesellschaft' and the 'Arbeitsgemeinschaft Oralpathologie' on "Oral Viral Infection and Oral Tumors" in Rostock, Germany on 6–7 July 2001.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ostwald, C., Rutsatz, K., Schweder, J. et al. Human papillomavirus 6/11, 16 and 18 in oral carcinomas and benign oral lesions. Med Microbiol Immunol 192, 145–148 (2003). https://doi.org/10.1007/s00430-002-0161-y

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00430-002-0161-y

Keywords.

Search

Navigation