Abstract
Neurons producing melanin-concentrating hormone (MCH) are located in the tuberal lateral hypothalamus (LHA) and in the rostromedial part of the zona incerta (ZI). This distribution suggests that rostromedial ZI shares some common features with the LHA. However, its functions with regard to arousal or feeding, which are often associated with the LHA, have not been thoroughly investigated. This study analyses the responses in the tuberal LHA and adjacent rostromedial ZI after experiments related to arousal, exploration, food teasing and ingestive behavior. Specific aspects of the connections of the rostromedial ZI were also studied using retrograde and anterograde tract-tracing approaches. The rostromedial ZI is activated during exploratory and teasing experiments. It receives specific projections from the frontal eye field and the anterior pole of the superior colliculus that are involved in gaze fixation and saccadic eye movements. It also receives projections from the laterodorsal tegmental nucleus involved in attention/arousal. By contrast, the tuberal LHA is activated during wakefulness and exploratory behavior and reportedly receives projections from the medial prefrontal and insular cortex, and from several brainstem structures such as the periaqueductal gray. We conclude that the rostromedial ZI is involved in attentional processes while the adjacent tuberal LHA is involved in arousal.
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Abbreviations
- ACAd:
-
Anterior cingulate area, dorsal part
- ACB:
-
Nucleus accumbens
- ac:
-
Anterior commissure
- aco:
-
Anterior commissure, olfactory limb
- AD:
-
Anterodorsal nucleus thalamus
- AHN:
-
Anterior hypothalamic nucleus
- AId:
-
Agranular insular area, dorsal part
- AM:
-
Anteromedial nucleus thalamus
- AMd:
-
Anteromedial nucleus thalamus, dorsal part
- AP:
-
Anteroposterior
- APN:
-
Anterior pretectal nucleus
- AQ:
-
Cerebral aqueduct proper
- ARH:
-
Arcuate hypothalamic nucleus
- AV:
-
Anteroventral nucleus thalamus
- BLA:
-
Basolateral amygdalar nucleus
- BST:
-
Bed nuclei stria terminalis
- C:
-
Control rat
- CEA:
-
Central amygdalar nucleus
- CL:
-
Central lateral nucleus thalamus
- CLA:
-
Claustrum
- CM:
-
Central medial nucleus thalamus
- CP:
-
Caudoputamen
- cpd:
-
Cerebral peduncle
- CRH:
-
Corticotropin-releasing hormone
- CS:
-
Superior central nucleus raphé
- cst:
-
Corticospinal tract
- CUN:
-
Cuneiform nucleus
- DAB:
-
Diaminobenzidine
- DMH:
-
Dorsomedial hypothalamic nucleus
- DMHa:
-
Dorsomedial hypothalamic nucleus, anterior part
- DMHp:
-
Dorsomedial hypothalamic nucleus, posterior part
- DMHv:
-
Dorsomedial hypothalamic nucleus, ventral part
- DR:
-
Dorsal nucleus raphé
- DV:
-
Dorsoventral
- fa:
-
Corpus callosum, anterior forceps
- FEF:
-
Frontal eye field
- FF:
-
Fields of Forel
- FG:
-
Fluorogold
- FL:
-
Flocculus
- fr:
-
Fasciculus retroflexus
- fx:
-
Columns of the fornix
- GABA:
-
Gamma-aminobutyric acid
- GP:
-
Globus pallidus
- GU:
-
Gustatory area
- Hcrt:
-
Hypocretin
- HRP:
-
Horseradish peroxidase
- ICe:
-
Inferior colliculus, external nucleus
- ILA:
-
Infralimbic area
- IMD:
-
Intermediodorsal nucleus thalamus
- int:
-
Internal capsule
- IP:
-
Intraperitoneal
- IPN:
-
Interpeduncular nucleus
- LD:
-
Lateral dorsal nucleus thalamus
- LDT:
-
Laterodorsal tegmental nucleus
- LG:
-
Lateral geniculate complex
- LH:
-
Lateral habenula
- LHA:
-
Lateral hypothalamic area
- LM:
-
Lateral mammillary nucleus
- LP:
-
Lateral posterior nucleus thalamus
- MA:
-
Magnocellular nucleus
- MCH:
-
Melanin-concentrating hormone
- MD:
-
Mediodorsal nucleus thalamus
- ME:
-
Median eminence
- MG:
-
Medial geniculate complex
- ML:
-
Mediolateral
- MM:
-
Medial mammillary nucleus, body
- MOp:
-
Primary somatomotor area
- MOs:
-
Secondary somatomotor areas
- MPN:
-
Medial preoptic nucleus
- MPT:
-
Medial pretectal area
- MRN:
-
Midbrain reticular nucleus
- mtt:
-
Mammillothalamic tract
- N:
-
Naïve rat
- Nc:
-
Cage naïve rat
- NLL:
-
Nucleus of the lateral lemniscus
- Nn:
-
Night naïve rat
- NOT:
-
Nucleus of the optic tract
- NPC:
-
Nucleus of the posterior commissure
- opt:
-
Optic tract
- OT:
-
Olfactory tubercle
- PAG:
-
Periaqueductal gray
- PB:
-
Parabrachial nucleus
- PBS:
-
Phosphate buffered saline
- PBS-T:
-
Phosphate buffered saline with triton
- PF:
-
Parafascicular nucleus
- PFA:
-
Paraformaldehyde
- PG:
-
Pontine gray
- PH:
-
Posterior hypothalamic nucleus
- PHA-L:
-
Phaseolus vulgaris leucoagglutinin
- PIR:
-
Piriform area
- PL:
-
Prelimbic area
- pm:
-
Principal mammillary tract
- PMd:
-
Dorsal premammillary nucleus
- PMv:
-
Ventral premammillary nucleus
- PO:
-
Posterior complex thalamus
- POA:
-
Preoptic area
- PP:
-
Peripeduncular nucleus
- PPN:
-
Pedunculopontine nucleus
- PPT:
-
Posterior pretectal nucleus
- PR:
-
Perireuniens nucleus
- PRC:
-
Precommissural nucleus, periaqueductal gray
- PRN:
-
Pontine reticular nucleus
- PSV:
-
Principal sensory nucleus of the trigeminal
- PVH:
-
Paraventricular hypothalamic nucleus
- PVT:
-
Paraventricular thalamic nucleus
- py:
-
Pyramid
- RE:
-
Nucleus reuniens
- REa:
-
Nucleus reuniens, rostral division, anterior part
- RH:
-
Rhomboid nucleus
- RL:
-
Rostral linear nucleus raphé
- RM:
-
Nucleus raphé magnus
- RN:
-
Red nucleus
- ROI:
-
Region of interest
- RR:
-
Midbrain reticular nucleus, retrorubral area
- RS:
-
Re-fed rat
- RT:
-
Reticular nucleus thalamus
- SC:
-
Superior colliculus
- SCH:
-
Suprachiasmatic nucleus
- SCig:
-
Superior colliculus, intermediate gray layer
- SF:
-
Septofimbrial nucleus
- SI:
-
Substantia innominata
- sm:
-
Stria medullaris
- SMT:
-
Submedial nucleus thalamus
- SN:
-
Substantia nigra
- SNr:
-
Substantia nigra, reticular part
- SSp:
-
Primary somatosensory area
- STN:
-
Subthalamic nucleus
- SUM:
-
Supramammillary nucleus
- SUMl:
-
Supramammillary nucleus, lateral part
- T:
-
Teased rat
- TT:
-
Tenia tecta
- V3:
-
Third ventricle
- V4:
-
Fourth ventricle
- VAL:
-
Ventral anterior-lateral complex thalamus
- VL:
-
Lateral ventricle
- vlt:
-
Ventrolateral tract
- VM:
-
Ventral medial nucleus thalamus
- VMH:
-
Ventromedial hypothalamic nucleus
- VPL:
-
Ventral posterolateral nucleus thalamus
- VPM:
-
Ventral posteromedial nucleus thalamus
- VTA:
-
Ventral tegmental area
- ZI:
-
Zona incerta
- ZIda:
-
Zona incerta, dopaminergic group
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Acknowledgements
This research was supported by funding from the French Ministère de la Recherche et de la Technologie (EA 3922) and from the Région de Franche-Comté. The authors want to thank Frances Thivet for the help in the preparation of the manuscript.
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Movie S1 During the feeding procedure, the teased rat previously deprived of food during 24h spent most of the one hour experiment trying to reach the food inside the meshed box while the control rat rested in the cage. (MOV 2258 KB)
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Chometton, S., Charrière, K., Bayer, L. et al. The rostromedial zona incerta is involved in attentional processes while adjacent LHA responds to arousal: c-Fos and anatomical evidence. Brain Struct Funct 222, 2507–2525 (2017). https://doi.org/10.1007/s00429-016-1353-3
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DOI: https://doi.org/10.1007/s00429-016-1353-3