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Local corticotropin releasing hormone (CRH) signals to its receptor CRHR1 during postnatal development of the mouse olfactory bulb

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Abstract

Neuropeptides play important physiological functions during distinct behaviors such as arousal, learning, memory, and reproduction. However, the role of local, extrahypothalamic neuropeptide signaling in shaping synapse formation and neuronal plasticity in the brain is not well understood. Here, we characterize the spatiotemporal expression profile of the neuropeptide corticotropin-releasing hormone (CRH) and its receptor CRHR1 in the mouse OB throughout development. We found that CRH-expressing interneurons are present in the external plexiform layer, that its cognate receptor is expressed by granule cells, and show that both CRH and CRHR1 expression enriches in the postnatal period when olfaction becomes important towards olfactory-related behaviors. Further, we provide electrophysiological evidence that CRHR1-expressing granule cells functionally respond to CRH ligand, and that the physiological circuitry of CRHR1 knockout mice is abnormal, leading to impaired olfactory behaviors. Together, these data suggest a physiologically relevant role for local CRH signaling towards shaping the neuronal circuitry within the mouse OB.

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Acknowledgments

We would like to thank Dr. Roy Sillitoe for critical comments on this manuscript. This work was supported through the McNair Medical Institute, and NINDS awards 1F31NS081805 to I.G. and 1R01NS078294 to B.R.A. The project described was supported in part by the RNA in situ Hybridization Core facility at Baylor College of Medicine, which is supported by a shared instrumentation Grant from the NIH (1S10OD016167) and the NIH IDDRC Grant 5P30HD024064 from the Eunice Kennedy Shriver National Institute of Child Health and Human Development.

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  1. Program in Developmental Biology, Baylor College of Medicine, Houston, TX, 77030, USA

    Isabella Garcia, Burak Tepe, Joshua Ortiz-Guzman, Alexander M. Herman, Lesley Chaboub, Benjamin Deneen & Benjamin R. Arenkiel

  2. Medical Scientist Training Program, Baylor College of Medicine, Houston, TX, 77030, USA

    Isabella Garcia

  3. Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, 77030, USA

    Paramjit K. Bhullar & Benjamin R. Arenkiel

  4. Department of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA

    Longwen Huang, Benjamin Deneen & Benjamin R. Arenkiel

  5. Institute of Molecular Medicine, University of Texas Health Science Center at Houston, Houston, TX, 77030, USA

    Nicholas J. Justice

  6. The Jan and Dan Duncan Neurological Research Institute at Texas Children’s Hospital, Houston, TX, 77030, USA

    Benjamin Deneen & Benjamin R. Arenkiel

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  1. Isabella Garcia
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Correspondence to Benjamin R. Arenkiel.

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Garcia, I., Bhullar, P.K., Tepe, B. et al. Local corticotropin releasing hormone (CRH) signals to its receptor CRHR1 during postnatal development of the mouse olfactory bulb. Brain Struct Funct 221, 1–20 (2016). https://doi.org/10.1007/s00429-014-0888-4

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