Abstract
Repetitive transcranial magnetic stimulation (rTMS) of the human brain can lead to long-lasting changes in cortical excitability. However, the cellular and molecular mechanisms which underlie rTMS-induced plasticity remain incompletely understood. Here, we used repetitive magnetic stimulation (rMS) of mouse entorhino-hippocampal slice cultures to study rMS-induced plasticity of excitatory postsynapses. By employing whole-cell patch-clamp recordings of CA1 pyramidal neurons, local electrical stimulations, immunostainings for the glutamate receptor subunit GluA1 and compartmental modeling, we found evidence for a preferential potentiation of excitatory synapses on proximal dendrites of CA1 neurons (2–4 h after stimulation). This rMS-induced synaptic potentiation required the activation of voltage-gated sodium channels, L-type voltage-gated calcium channels and N-methyl-d-aspartate-receptors. In view of these findings we propose a cellular model for the preferential strengthening of excitatory synapses on proximal dendrites following rMS in vitro, which is based on a cooperative effect of synaptic glutamatergic transmission and postsynaptic depolarization.
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Acknowledgments
We thank Charlotte Nolte-Uhl and Nadine Zahn for their excellent assistance in tissue culturing. The work was supported by Deutsche Forschungsgemeinschaft (CRC1080 and FOR 1332 to T.D. and A.V.).
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M. Lenz, S. Platschek and V. Priesemann have contributed equally to this work.
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Lenz, M., Platschek, S., Priesemann, V. et al. Repetitive magnetic stimulation induces plasticity of excitatory postsynapses on proximal dendrites of cultured mouse CA1 pyramidal neurons. Brain Struct Funct 220, 3323–3337 (2015). https://doi.org/10.1007/s00429-014-0859-9
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DOI: https://doi.org/10.1007/s00429-014-0859-9