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Distribution of SMI-32-immunoreactive neurons in the central auditory system of the rat

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Abstract

SMI-32 antibody recognizes a non-phosphorylated epitope of neurofilament proteins, which are thought to be necessary for the maintenance of large neurons with highly myelinated processes. We investigated the distribution and quantity of SMI-32-immunoreactive(-ir) neurons in individual parts of the rat auditory system. SMI-32-ir neurons were present in all auditory structures; however, in most regions they constituted only a minority of all neurons (10–30%). In the cochlear nuclei, a higher occurrence of SMI-32-ir neurons was found in the ventral cochlear nucleus. Within the superior olivary complex, SMI-32-ir cells were particularly abundant in the medial nucleus of the trapezoid body (MNTB), the only auditory region where SMI-32-ir neurons constituted an absolute majority of all neurons. In the inferior colliculus, a region with the highest total number of neurons among the rat auditory subcortical structures, the percentage of SMI-32-ir cells was, in contrast to the MNTB, very low. In the medial geniculate body, SMI-32-ir neurons were prevalent in the ventral division. At the cortical level, SMI-32-ir neurons were found mainly in layers III, V and VI. Within the auditory cortex, it was possible to distinguish the Te1, Te2 and Te3 areas on the basis of the variable numerical density and volumes of SMI-32-ir neurons, especially when the pyramidal cells of layer V were taken into account. SMI-32-ir neurons apparently form a representative subpopulation of neurons in all parts of the rat central auditory system and may belong to both the inhibitory and excitatory systems, depending on the particular brain region.

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Acknowledgments

The authors wish to thank Mrs. J. Janouskova for her technical assistance. This study was supported by the Grant Agency of the Czech Republic (309/07/1336), AV0Z50390512 and LC 554.

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  1. Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Prague, Czech Republic

    Ladislav Ouda & Josef Syka

  2. Department of Anatomy, 1st and 2nd Medical Faculty, Charles University, Prague, Czech Republic

    Rastislav Druga

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  1. Ladislav Ouda
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Ouda, L., Druga, R. & Syka, J. Distribution of SMI-32-immunoreactive neurons in the central auditory system of the rat. Brain Struct Funct 217, 19–36 (2012). https://doi.org/10.1007/s00429-011-0329-6

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