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Adhesion activity of fetal gonadal cells to EGF and discoidin domains of milk fat globule-EGF factor 8 (MFG-E8), a secreted integrin-binding protein which is transiently expressed in mouse early gonadogenesis

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Abstract

MFG-E8, a secreted integrin-binding protein, consists of two EGF domains containing a RGD motif and two discoidin domains. In mouse embryogenesis, MFG-E8 is highly expressed in gonadal stromal cells near mesonephros at 11.5–12.5 dpc, but its function in gonadogenesis has not been characterized. To clarify a possible role of MFG-E8 in developing gonads, we analyzed the adhesion activity of 10.5–15.5 dpc gonadal cells to recombinant proteins of EGF or discoidin domains of MFG-E8. In EGF-coated wells, the gonadal cells at 11.5–12.5 dpc revealed a significantly higher adhesion activity as compared to those at 10.5 and 15.5 dpc, while discoidin domains showed a constant number of the adhered cells throughout these stages. To identify the adhesive cells of 11.5-dpc gonads, immunohistochemistry with anti-SF1/Ad4Bp antibody (a specific marker for supporting, steroidogenic, and coelomic epithelial cells) and staining for alkaline phosphatase (a germ cell marker) were carried out. As a result, EGF domains, as well as discoidin domains, were capable of binding to all three groups of SF1/Ad4Bp-positive and negative somatic cells, and germ cells of 11.5-dpc gonads. These findings therefore suggest that MFG-E8 mediates the cell-to-cell interaction among several somatic cell types and germ cells in mouse early gonadogenesis.

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Acknowledgements

We are extremely grateful to Dr. Kuniko Furutachi-Nakano and Mr. Iwao Tsugiyama for their secretarial and technical supports. This work was supported by Grant-in Aids for Scientific Research Fund from the Ministry of Education, Science, Sports and Culture of Japan (to Y. Kanai) and from Skylark Food Science Institute (to Y. Kanai).

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Authors and Affiliations

  1. Department of Veterinary Anatomy, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan

    Maki Ishii, Yoshiakira Kanai, Tay Tat Wei, Tomohide Kidokoro & Masamichi Kurohmaru

  2. Department of Anatomy, Kyorin University School of Medicine, Shinkawa 6-20-2, Mitaka, Tokyo, 181-8611, Japan

    Masami Kanai-Azuma

  3. Department of Biochemical Cell Research, The Tokyo Metropolitan Institute of Medical Science, Honkomagome 3-18-22, Bunkyo-ku, Tokyo, 113-8613, Japan

    Youichi Tajima & Yutaka Sanai

  4. Department of Global Animal Science, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan

    Yoshihiro Hayashi

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  1. Maki Ishii
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Ishii, M., Kanai, Y., Kanai-Azuma, M. et al. Adhesion activity of fetal gonadal cells to EGF and discoidin domains of milk fat globule-EGF factor 8 (MFG-E8), a secreted integrin-binding protein which is transiently expressed in mouse early gonadogenesis. Anat Embryol 209, 485–494 (2005). https://doi.org/10.1007/s00429-005-0463-0

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