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Heterogeneous occurrence of aquaporin-4 in the ependyma and in the circumventricular organs in rat and chicken

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Abstract

Aquaporins are selective water channel proteins critical in volume homeostasis. In the CNS AQP4 predominates, localized mainly in the glia limitans, the perivascular endfeet and ependyma. The present immunofluorescent study reveals the distribution of aquaporin-4 in the circumventricular organs in rat and chicken brains. The ventricular ependyma (especially in the third one), the subfornical organ, the area postrema, the rat pineal body (in part), and the vascular organ of lamina terminalis were marked by intense immunopositivity. Several areas, however, proved to be immunonegative: the central canal, the subcommissural organ, the ependymal zone of the median eminence in rat but its whole thickness in chicken, the subtrochlear organ, and the paraventricular organ. The immunostaining of the lateral septal and subseptal organs were similar to their environment. Results on developing rats suggested that the aquaporin-4 immunonegativity is a secondary phenomenon. Surveying other structural and functional features, no clear explanation of the heterogeneous occurrence of aquaporin-4 was found. The absence of aquaporin-4 seems to correlate with some features of the “ependymal organs” (thickened, pseudostratified ependyma, presence of blood–brain barrier) and with the avoidance of GFAP. On the other hand, the organs rich in aquaporin-4 have features of the “hypendymal organs” (glial and vascular plexus but no blood–brain barrier). There are organs, however, which do not fit into either group completely, i.e. the lateral septal and subseptal organs. Presence of tight junctions coincides with the absence of aquaporin-4 in the ependyma of spinal cord, the subcommissural organ and the ependyma of median eminence.

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Abbreviations

AQP4:

Aquaporin-4

CVO:

Circumventricular organ

GFAP:

Glial fibrillary acidic protein

LSO:

Lateral septal organ

OVLT:

Vascular organ of lamina terminalis (organon vasculosum laminae terminalis)

PVO:

Paraventricular organ

SCO:

Subcommissural organ

SFO:

Subfornical organ

STO:

Subtrochlear organ

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Acknowledgements

This study was supported by the Department of Anatomy, Histology and Embryology and the PhD School of the Semmelweis University. The technical assistance of Ms. S. Deák and Ms. E. Oszwald is highly appreciated.

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  1. Department of Anatomy, Histology and Embryology, Semmelweis University of Medicine, Tűzoltó 58, 1094, Budapest, Hungary

    Oded Goren, István Adorján & Mihály Kálmán

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  1. Oded Goren
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Goren, O., Adorján, I. & Kálmán, M. Heterogeneous occurrence of aquaporin-4 in the ependyma and in the circumventricular organs in rat and chicken. Anat Embryol 211, 155–172 (2006). https://doi.org/10.1007/s00429-005-0067-8

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