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The ontogeny of mucosal immune cells in common carp (Cyprinus carpio L.)

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Abstract

The ontogeny of carp (Cyprinus carpio L.) immune cells was studied in mucosal organs (intestine, gills and skin) using the monoclonal antibodies WCL38 (intraepithelial lymphocytes), WCL15 (monocytes/macrophages) and WCI12 (B cells). In addition, recombination activating gene 1 expression was examined in the intestine with real time quantitative PCR and in situ hybridization to investigate extrathymic generation of lymphocytes. WCL38+ intraepithelial lymphocytes (putative T cells) appeared in the intestine at 3 days post-fertilization (dpf), which is shortly after hatching but before feeding, implying an important function at early age. These lymphoid cells appear in the intestine before the observation of the first thymocytes at 3–4 dpf, and together with the expression of recombination activating gene 1 in the intestine, suggests that similar to mammals at least part of these cells are generated in the intestine. WCL15+ monocytes/macrophages appeared in the lamina propria of the intestine at 7 dpf, but considerably later in the epithelium, while WCI12+ (B) cells appeared in intestine and gills at 6–7 weeks. From these results it can be concluded that putative T cells occur much earlier than B cells, and that B cells appear much later in the mucosae than in other internal lymphoid organs (2 wpf).

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Abbreviations

dpf:

Days post-fertilization

IEL:

Intraepithelial lymphocytes

Ig:

Immunoglobulin

MAb:

Monoclonal antibody

PBL:

Peripheral blood leucocytes

rag :

Recombination activating gene

RQ-PCR:

Real time quantitative polymerase chain reaction

TCR:

T cell receptor

wpf:

weeks post-fertilization

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Acknowledgements

The authors wish to thank Mrs. T. van der Wal and Mr. S. Leenstra for providing carp, and Prof. Dr H.F.J. Savelkoul for critically reading the manuscript. This work was supported by the Italian National Research Council (CNR 1998, A.I. n.98.00194) and the European Union (QLK2-CT-2000-01076).

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Authors and Affiliations

  1. Cell Biology and Immunology Group, Wageningen Institute of Animal Sciences, Wageningen University, Marijkeweg 40, 6709 PG, Wageningen, The Netherlands

    Heidi B.T. Huttenhuis, Carolien N. Van Oosterhoud, Anja J. Taverne-Thiele, Willem B. Van Muiswinkel & Jan H.W.M. Rombout

  2. Department of Environmental Sciences, Functional Anatomy and Developmental Biology, Tuscia University, Viterbo, Italy

    Nicla Romano & Lucia Mastrolia

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  1. Heidi B.T. Huttenhuis
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  7. Jan H.W.M. Rombout
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Correspondence to Jan H.W.M. Rombout.

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Huttenhuis, H.B., Romano, N., Van Oosterhoud, C.N. et al. The ontogeny of mucosal immune cells in common carp (Cyprinus carpio L.). Anat Embryol 211, 19–29 (2006). https://doi.org/10.1007/s00429-005-0062-0

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  • DOI: https://doi.org/10.1007/s00429-005-0062-0

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