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Detection of βig-H3, a TGFβ induced gene, during cardiac development and its complementary pattern with periostin

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Abstract

Regulation of normal cardiac development involves numerous transcription factors, cytoskeletal proteins, signaling molecules, and extracellular matrix proteins. These key molecular components act in concert to induce morphological changes essential for the proper development of a functional four-chambered heart. Growth factors such as BMPs and TGFβ’s play a role in migration, proliferation and differentiation during cardiac development and are important regulators of the extracellular matrix (ECM). Genes responsive to these morphogens are likely to play an equally significant role during cardiac development. Therefore, we sought to clone the chicken TGFβ induced gene βig-H3 and evaluate its spatio-temporal expression during heart morphogenesis. Our studies show by Northern analysis, whole mount and section in situ hybridization experiments that βig-H3 is expressed primarily in the mesenchyme of the atrioventricular and outflow tract cushions and later in the right and left atrioventricular valve leaflets and supporting valve structures. The mRNA expression domains of βig-H3 show a complementary pattern compared to that of its highly homologous relative, periostin.

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Acknowledgments

The authors are grateful for the superb technical expertise of Sally Fairey. This project was supported by the National Heart, Lung and Blood Institutes of Health grants RO1-HL66231 (to C.H.M) and RO1-HL33756, PO1-HL52813 (to R.R.M. and A.W.), and a Pre-doctoral Cardiovascular Training Grant T32 HL07260 (E.E.W).

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  1. Department of Cell Biology and Anatomy, Medical University of South Carolina, 173 Ashley Ave BSB 642, Charleston, SC, 29425, USA

    Russell A. Norris, Christine B. Kern, Andy Wessels, Elaine E. Wirrig, Roger R. Markwald & Corey H. Mjaatvedt

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  1. Russell A. Norris
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  2. Christine B. Kern
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  3. Andy Wessels
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  4. Elaine E. Wirrig
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  6. Corey H. Mjaatvedt
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Correspondence to Corey H. Mjaatvedt.

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Norris, R.A., Kern, C.B., Wessels, A. et al. Detection of βig-H3, a TGFβ induced gene, during cardiac development and its complementary pattern with periostin. Anat Embryol 210, 13–23 (2005). https://doi.org/10.1007/s00429-005-0010-z

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