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Tumor-associated macrophages are involved in tumor progression in papillary renal cell carcinoma

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Abstract

Tumor-associated macrophages (TAMs) play a key role in cancer development. Especially, the immunosuppressive M2 phenotype is associated with increased tumor growth, invasiveness and metastasis. The differentiation of macrophages to the alternative phenotype M2 is mediated, inter alia, by macrophage colony-stimulating factor (M-CSF). Papillary renal cell carcinoma (RCC) represents a rare tumor type which, based upon histological criteria, can be subdivided into two subtypes (I and II), of which type II is associated with poor prognosis. In both subtypes, typically, a dense infiltrate of macrophages is found. In the present study, the expression of CD68, CD163, M-CSF, Ki-67, and CD31 was examined in 30 type I and 30 type II papillary RCCs (n = 60). Both types of papillary RCCs contained an equally dense infiltrate of CD68-positive macrophages. Nearly all macrophages in papillary RCC type II expressed CD163, a characteristic for M2 macrophages. In type I papillary RCC, less than 30 % of macrophages expressed CD163. Furthermore, tumor cells in type II papillary RCC expressed significantly more M-CSF and showed increased (Ki-67 expression defined) proliferative activity in comparison with type I papillary RCC. In addition, the (CD31 defined) capillary density was higher in type II than in type I papillary RCC. A dense infiltrate of M2 phenotype TAM and high M-CSF expression in tumor cells are key features of type II papillary RCC. These findings might explain why the prognosis of papillary RCC type II is worse than that of type I.

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Authors and Affiliations

  1. Department of Pathology, University of Göttingen, Robert-Koch-Str. 40, 37075, Göttingen, Germany

    Carl Ludwig Behnes, Felix Bremmer, Bernhard Hemmerlein, Philipp Ströbel & Heinz-Joachim Radzun

  2. Department of Urology, University of Göttingen, Robert-Koch-Str. 40, 37075, Göttingen, Germany

    Arne Strauss

  3. Department of Pathology, Krefeld, Germany

    Bernhard Hemmerlein

  4. Department of Pathology, University of Göttingen, Robert-Koch-Str. 40, 37083, Göttingen, Germany

    Carl Ludwig Behnes

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  1. Carl Ludwig Behnes
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Correspondence to Carl Ludwig Behnes.

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Behnes, C.L., Bremmer, F., Hemmerlein, B. et al. Tumor-associated macrophages are involved in tumor progression in papillary renal cell carcinoma. Virchows Arch 464, 191–196 (2014). https://doi.org/10.1007/s00428-013-1523-0

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