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Frequent loss of heterozygosity on chromosome 12q in non-small-cell lung carcinomas

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Abstract

Chromosomal aberrations in non-small-cell lung carcinomas (NSCLCs) are common events. In our study, the lung cancer cell lines (NCI-H446 and SPC-A-1) displayed numerous numerical and structural alterations in their chromosomes by G-banded karyotypic analysis, and abnormalities of chromosome 12 by fluorescence in situ hybridization. Sequentially, we used 14 microsatellite markers within 12q to analyze loss of heterozygosity (LOH) in lung cancer cell lines and NSCLCs. Possible LOH on 12q were statistically inferred to occur in five lung cell lines. Importantly, 17 out of 25 NSCLCs (68%) showed LOH at chromosome 12q. Frequencies of LOH for individual markers ranged from 18% to 44%. Several deletions which were marked with D12S1301, D12S2196, D12S398, D12S90, D12S1056, D12S1713, D12S375, D12S1040, D12S326, and D12S106 were newly detected. Allelic loss on 12q15–q21 detected with D12S1040 occurred at the later stages of NSCLC progression (p < 0.05, Fisher’s exact test). LOH on 12q marked with D12S2196, D12S398, D12S326, and D12S106 were frequently found in NSCLCs from the patients without smoking history (p < 0.05, Fisher’s exact test). These findings indicated that allelic loss on 12q is commonly involved in NSCLCs, and new tumor suppressor genes may occur within 12q.

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Acknowledgment

We thank the colleagues from the Department of Surgery of Yunnan First People Hospital for providing surgical specimens. Supported by Hundred Talents Program (Grant Cao) and West Light Foundation (Grant Liang) of Chinese Academy of Sciences.

Conflict of interest statement

We declare that we have no conflict of interest.

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Authors and Affiliations

  1. Key Laboratory of Animal Models and Human Disease Mechanisms of CAS and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 Jiaochang Donglu, Kunming, Yunnan, 650223, China

    Xiao-Jie Ding, Min-Xia Liu, Lei Ao, Yi-Rong Liang & Yi Cao

  2. Graduate University of the Chinese Academy of Sciences, Beijing, 100049, China

    Min-Xia Liu

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  1. Xiao-Jie Ding
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  2. Min-Xia Liu
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  3. Lei Ao
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  4. Yi-Rong Liang
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  5. Yi Cao
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Correspondence to Yi Cao.

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Ding, XJ., Liu, MX., Ao, L. et al. Frequent loss of heterozygosity on chromosome 12q in non-small-cell lung carcinomas. Virchows Arch 458, 561–569 (2011). https://doi.org/10.1007/s00428-011-1042-9

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  • DOI: https://doi.org/10.1007/s00428-011-1042-9

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