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Expression of the GA733 gene family and its relationship to prognosis in pulmonary adenocarcinoma

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Abstract

The GA733 gene family is composed of GA733-1 (TROP2) and GA733-2 (Ep-CAM), whose expression has been examined in various carcinomas and reported to be significantly associated with prognosis. The aim of this study was to investigate the expression of GA733 gene family members and to compare their prognostic significance in pulmonary adenocarcinoma. One hundred thirty paraffin-embedded specimens of small-sized pulmonary adenocarcinoma, less than 2 cm in diameter, were categorized using the classification of small-sized pulmonary adenocarcinoma devised by Noguchi et al. (Cancer 75:2844–2852, 1995) and examined immunohistochemically using a murine monoclonal antibody against Ep-CAM and a goat polyclonal antibody against TROP2. The patient survival rate was calculated using the Kaplan–Meier method. Ep-CAM and TROP2 were similarly expressed in many small-sized pulmonary adenocarcinomas. The expression of Ep-CAM was significantly related to a favorable outcome (p = 0.0185), whereas TROP2 tended to be expressed in cases with an unfavorable outcome (p = 0.0564), and was significantly associated with an unfavorable outcome in nonlepidic-type adenocarcinomas (p = 0.0125). Multivariate analysis showed that TROP2 overexpression and lymph node metastasis were independent prognostic markers. Although the two GA733 proteins share structural similarity, they appear to have opposite biological significances in small-sized adenocarcinomas. As the expression of TROP2 was detected in more poorly differentiated tumors, the protein may have oncogenic activity.

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Acknowledgments

This work was supported in part by a Grant-in-Aid for Cancer Research (16-1) from the Ministry of Health, Labour and Welfare of Japan.

Conflict of interest statement

We declare that we have no conflict of interest.

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Authors and Affiliations

  1. Department of Pathology, Institute of Basic Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba-shi, Ibaraki, 305-8575, Japan

    Hiromi Kobayashi, Yuko Minami, Yoichi Anami, Junko Kano & Masayuki Noguchi

  2. Department of Pathology, Tsukuba University Hospital, Ibaraki, Japan

    Yuzuru Kondou & Yukio Morishita

  3. Division of Pathology, Ibaraki Prefectural Central Hospital, Ibaraki, Japan

    Tatsuo Iijima

  4. Clinical Laboratory Division, National Cancer Center Hospital, Tokyo, Japan

    Koji Tsuta

  5. Division of Pulmonary Diseases, Department of Internal Medicine, University of Saga, Saga, Japan

    Hiromi Kobayashi & Shinichiro Hayashi

Authors
  1. Hiromi Kobayashi
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  2. Yuko Minami
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  3. Yoichi Anami
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  4. Yuzuru Kondou
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  5. Tatsuo Iijima
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  6. Junko Kano
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  7. Yukio Morishita
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  8. Koji Tsuta
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  9. Shinichiro Hayashi
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  10. Masayuki Noguchi
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Correspondence to Masayuki Noguchi.

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Kobayashi, H., Minami, Y., Anami, Y. et al. Expression of the GA733 gene family and its relationship to prognosis in pulmonary adenocarcinoma. Virchows Arch 457, 69–76 (2010). https://doi.org/10.1007/s00428-010-0930-8

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  • DOI: https://doi.org/10.1007/s00428-010-0930-8

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