Abstract
The regenerating protein (Reg)-like protein (RELP, also known as Reg IV) is a recently characterized fourth member of the human Reg protein family. The Reg proteins are small, about 20-kD-sized, secretory proteins of C-lectin type. The previously known Reg proteins have been functionally implicated in regeneration, proliferation, and differentiation of the pancreas, liver, and gastrointestinal mucosa. To study the tissue expression of RELP, we raised a monoclonal antibody to RELP. By immunohistochemistry and in situ hybridization, we found a robust de novo expression of RELP in the neoplastic goblet cells of appendiceal mucinous cystadenomas and in the epithelial implants of pseudomyxoma peritonei (PMP). Our findings indicate that RELP serves as a marker for appendiceal mucinous cystadenomas and PMP, and that RELP may contribute to the pathogenesis of these disorders.
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Abbreviations
- RELP:
-
Reg-like protein
- Reg:
-
regenerating
- PMP:
-
pseudomyxoma peritonei
- EIA:
-
enzyme immunoassay
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Acknowledgements
We thank Jill Carton, Centocor, Inc for preparing the immunogen for mab generation and Anu Harju for technical assistance. This work was supported by The Finnish Academy of Sciences, The Finnish Cancer Foundation, Biocentrum Helsinki, and Finska Läkaresällskapet.The experiments reported in this manuscript comply with the current laws of Finland.
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Heiskala, K., Giles-Komar, J., Heiskala, M. et al. High expression of RELP (Reg IV) in neoplastic goblet cells of appendiceal mucinous cystadenoma and pseudomyxoma peritonei. Virchows Arch 448, 295–300 (2006). https://doi.org/10.1007/s00428-005-0105-1
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DOI: https://doi.org/10.1007/s00428-005-0105-1