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Monocyte chemotactic S19 ribosomal protein dimer in atherosclerotic vascular lesion

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Abstract

To elucidate the molecular mechanism inducing monocyte/macrophage infiltration in the atherosclerotic lesion, we measured the monocyte chemotactic capacity in the extracts of aortic lesions. Five out of seven extracts exhibited significant chemotactic activities. Immunohistochemical examination with an anti-CD68 monoclonal antibody demonstrated that the five positive lesions possessed obvious monocyte/macrophage infiltrations at the intima, whereas the two negative lesions did so at significantly lower intensities. We subjected the chemotactic extracts to immunological analyses to identify the monocyte chemoattractant in them. The monocyte chemotactic capacities of all positive extracts were removed with anti-S19 ribosomal protein (RP S19) antibody beads and antimonocyte chemoattractant protein-1 (MCP-1) antibody beads. In three of the five extracts, the anti-RP S19 antibody beads were more effective than the anti-MCP-1 antibody beads for removal, while in the remaining two extracts, the opposite was observed. A combined immunoabsorption with these beads depleted the monocyte chemotactic capacity of a representative sample of each group. Consistently, the chemotactic capacity of an apparently RP S19 dimer-predominant extract was strongly inhibited by the presence of a C5a receptor antagonist. These results suggest that the RP S19 dimer and MCP-1 play a major role in the monocyte/macrophage infiltration of the atherosclerotic vascular lesion.

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Acknowledgements

The authors are grateful to Prof. M. Takeya and Miss T. Kubo of Faculty of Medical and Pharmaceutical Sciences, Kumamoto University for helpful suggestions and technical advice, respectively.

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Authors and Affiliations

  1. Department of Molecular Pathology, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, 2-2-1 Honjo, Kumamoto, 860-0811, Japan

    Lei Shi, Kazutaka Tokita & Tetsuro Yamamoto

  2. Department of Cardiovascular Surgery, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, 2-2-1 Honjo, Kumamoto, 860-0811, Japan

    Shigeyuki Tsurusaki, Ryuji Kunitomo & Michio Kawasuji

  3. Trans Genic Incorporation, Kumamoto, Japan

    Noriko Futa, Tamami Sakamoto & Tomoko Matsuda

  4. Department of Biological Functions and Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Kitakyushu, Japan

    Norikazu Nishino

Authors
  1. Lei Shi
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  2. Shigeyuki Tsurusaki
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  3. Noriko Futa
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  4. Tamami Sakamoto
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  5. Tomoko Matsuda
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  6. Norikazu Nishino
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  7. Ryuji Kunitomo
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  8. Michio Kawasuji
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  9. Kazutaka Tokita
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  10. Tetsuro Yamamoto
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Corresponding author

Correspondence to Kazutaka Tokita.

Additional information

L. Shi and S. Tsurusaki contributed equally to this work.

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Shi, L., Tsurusaki, S., Futa, N. et al. Monocyte chemotactic S19 ribosomal protein dimer in atherosclerotic vascular lesion. Virchows Arch 447, 747–755 (2005). https://doi.org/10.1007/s00428-005-0012-5

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  • DOI: https://doi.org/10.1007/s00428-005-0012-5

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