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Spatiotemporal changes of coxsackievirus and adenovirus receptor in rat hearts during postnatal development and in cultured cardiomyocytes of neonatal rat

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Abstract

Coxsackievirus B is the most common cause of viral myocarditis and is particularly virulent in neonates and children. Adenovirus is also a leading cause of the disease. The determinant of tropism for both viruses is considered to be the expression of coxsackievirus and adenovirus receptor (CAR) in target organs. However, developmental change and physiological localization of CAR in the heart are unknown. We examined expression levels of CAR in rat hearts by quantitative real-time polymerase chain reaction and Western blot analysis and found that CAR decreased gradually during postnatal development, although CAR was detectable, even in adults. Immunohistochemistry revealed CAR on the whole surface of cardiomyocytes in immature rat hearts. In contrast, CAR was detected predominantly on intercalated disks in the adult heart and was accumulated especially at the contact point between the cultured cardiomyocytes, even though they were prepared from the neonatal rat heart. In conclusion, CAR was expressed abundantly on the whole surface of cardiomyocytes in immature rat hearts. Both the expression level and the localization of CAR are possible determinants of the susceptibility to viral myocarditis of neonates and children.

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Acknowledgement

This study was supported in part by a grant for research on specific diseases from the Ministry of Health, Labor and Welfare.

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Authors and Affiliations

  1. Division of Cardiology, Niigata University Graduate School of Medical and Dental Sciences, 1-754 Asahimachi, 951-8510, Niigata, Japan

    Takeshi Kashimura, Makoto Kodama, Kaori Yoshida, Takuya Ozawa, Ritsuo Watanabe, Yuji Okura, Kiminori Kato, Haruo Hanawa & Yoshifusa Aizawa

  2. Genome Science Branch, Center for Bioresource-Based Researches, Brain Research Institute, Niigata University, Niigata, Japan

    Yuko Hotta, Junichi Hosoya & Ryozo Kuwano

Authors
  1. Takeshi Kashimura
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  2. Makoto Kodama
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  3. Yuko Hotta
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Correspondence to Takeshi Kashimura.

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Kashimura, T., Kodama, M., Hotta, Y. et al. Spatiotemporal changes of coxsackievirus and adenovirus receptor in rat hearts during postnatal development and in cultured cardiomyocytes of neonatal rat. Virchows Arch 444, 283–292 (2004). https://doi.org/10.1007/s00428-003-0925-9

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  • DOI: https://doi.org/10.1007/s00428-003-0925-9

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