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Sequence analysis and high-throughput immunhistochemical profiling of KIT (CD 117) expression in uveal melanoma using tissue microarrays

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Abstract

We aimed to immunohistochemically examine the expression of KIT (CD 117) in human posterior uveal melanoma and to analyze KIT-positive tumors for gene mutations. Brought into a tissue microarray (TMA) format were 101 formalin-fixed, paraffin-embedded posterior uveal melanomas. Immunhistochemistry was performed using the polyclonal anti-CD117 antibody from Dako (A4502). In ten selected KIT-positive tumors, exons 2, 8, 9, 11, 13 and 17 were sequenced. Of the 101 cases, 89 (88%) could be evaluated on the TMAs. Immunohistochemistry for CD 117 was weakly positive in 5 cases (6%), moderately positive in 10 cases (12%) and strongly positive in 57 cases (69%). No KIT mutations were detected in the analyzed exons. In conclusion, human posterior uveal melanoma frequently expresses CD117 at high levels. Although KIT mutations could not be found, it appears justified to investigate the utility of imatinib mesylate in the treatment of these patients.

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Acknowledgement

The authors thank Miriam Vonlanten and Andreas Meyer for their technical assistance.

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Authors and Affiliations

  1. University Eye Clinic of Basel, Basel, Switzerland

    Mona Pache, Doris Bösch, Josef Flammer & Peter Meyer

  2. Institute of Pathology, University of Basel, Basel, Switzerland

    Katharina Glatz, Doris Bösch, Stephan Dirnhofer, Martina Mirlacher, Ronald Simon, Peter Schraml, Alex Rufle, Guido Sauter & Peter Meyer

  3. University Eye Clinic Basel, Mittlere Str. 91, 4056 , Basel, Switzerland

    Peter Meyer

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  1. Mona Pache
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  2. Katharina Glatz
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  3. Doris Bösch
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  5. Martina Mirlacher
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  6. Ronald Simon
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  10. Guido Sauter
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  11. Peter Meyer
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Correspondence to Peter Meyer.

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Pache, M., Glatz, K., Bösch, D. et al. Sequence analysis and high-throughput immunhistochemical profiling of KIT (CD 117) expression in uveal melanoma using tissue microarrays. Virchows Arch 443, 741–744 (2003). https://doi.org/10.1007/s00428-003-0883-2

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  • DOI: https://doi.org/10.1007/s00428-003-0883-2

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