Abstract
The larval shell emerges early in embryogenesis of mollusks, but the detailed mechanisms of its biogenesis remain to be determined. In this study, we cloned a tyrosinase gene (cgi-tyr1) that potentially functioned in larval shell biogenesis from the Pacific oyster Crassostrea gigas, a worldwide bivalve species. Sequence analysis of cgi-tyr1 revealed that it had typical copper-binding domains and a signal peptide. Through whole mount in situ hybridization and an electron scanning microscopic observation, we detected the expression of cgi-tyr1 firstly in the saddle-shaped shell field in trochophores, indicating that cgi-tyr1 might participate in the biogenesis of the initial non-calcified shell of trochophores. In the following development to early D-veliger, cells in the central region of shell field exhibited no detectable cgi-tyr1 expression, and cgi-tyr1 expression was sustained only in the edge of the shell field and the hinge region, indicating that cgi-tyr1 might function fundamentally in shell growth from trochophore to early D-veliger. Unexpectedly, cgi-tyr1 expression was not detected after the D-veliger stage. This indicated that other molecules might function in later shell development. Our results suggested a role for a tyrosinase gene that specifically functioned in the initial phase of the larval shell biogenesis of C. gigas. This work would shed light on future studies on larval shell development and might be helpful to understand how the molluscan shell emerged during evolution.
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References
Andersen SO (2010) Insect cuticular sclerotization: a review. Insect Biochem Mol Biol 40(3):166–178
Brown C (1950) Quinone tanning in the animal kingdom. Nature 165:275
Chang T-S (2009) An updated review of tyrosinase inhibitors. Int J Mol Sci 10(6):2440–2475
Checa A (2000) A new model for periostracum and shell formation in Unionidae (Bivalvia, Mollusca). Tissue Cell 32(5):405–416
Fang D, Xu G, Hu Y, Pan C, Xie L, Zhang R (2011) Identification of genes directly involved in shell formation and their functions in pearl oyster, Pinctada fucata. PLoS One 6(7):e21860
García-Borrón JC, Solano F (2002) Molecular anatomy of tyrosinase and its related proteins: beyond the histidine–bound metal catalytic center. Pigment Cell Res 15(3):162–173
Huan P, Wang H, Dong B, Liu B (2012) Identification of differentially expressed proteins involved in the early larval development of the Pacific oyster Crassostrea gigas. J Proteomics 75(13):3855–3865
Kin K, Kakoi S, Wada H (2009) A novel role for dpp in the shaping of bivalve shells revealed in a conserved molluscan developmental program. Dev Biol 329(1):152–166
LaBarbera M (1974) Calcification of the first larval shell of Tridacna squamosa (Tridacnidae: Bivalvia). Mar Biol 25(3):233–238
Marin F, Luquet G (2004) Molluscan shell proteins. Comptes Rendus Palevol 3(6):469–492
Maures TJ, Duan C (2002) Structure, developmental expression, and physiological regulation of zebrafish IGF binding protein-1. Endocrinology 143(7):2722–2731
Mouëza M, Gros O, Frenkiel L (2006) Embryonic development and shell differentiation in Chione cancellata (Bivalvia, Veneridae): an ultrastructural analysis. Invertebr Biol 125(1):21–33
Nagai K, Yano M, Morimoto K, Miyamoto H (2007) Tyrosinase localization in mollusk shells. Comp Biochem Physiol B-Biochem Mol Biol 146(2):207–214
Nederbragt AJ, van Loon AE, Dictus WJAG (2002) Expression of Patella vulgata orthologs of engrailed and dpp-BMP2/4 in adjacent domains during molluscan shell development suggests a conserved compartment boundary mechanism. Dev Biol 246(2):341–355
Zhang C, Xie L, Huang J, Chen L, Zhang R (2006) A novel putative tyrosinase involved in periostracum formation from the pearl oyster (Pinctada fucata). Biochem Biophys Res Commun 342(2):632–639
Zhang G, Fang X, Guo X, Li L, Luo R, Xu F, Yang P, Zhang L, Wang X, Qi H (2012) The oyster genome reveals stress adaptation and complexity of shell formation. Nature 490:49–54
Acknowledgments
This work was financially supported by project 31001102 supported by NSFC and the National Basic Research Program of China (2010CB126403).
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Communicated by David A Weisblat
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Huan, P., Liu, G., Wang, H. et al. Identification of a tyrosinase gene potentially involved in early larval shell biogenesis of the Pacific oyster Crassostrea gigas . Dev Genes Evol 223, 389–394 (2013). https://doi.org/10.1007/s00427-013-0450-z
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DOI: https://doi.org/10.1007/s00427-013-0450-z