Abstract
We have identified two Tbx1/10-related genes, LjTbx1/10A and LjTbx1/10B, from the Japanese river lamprey Lethenteron japonicum. We used in situ hybridization to characterize their expression pattern during embryonic development. LjTbx1/10A and LjTbx1/10B shared common expression in the pharyngeal arches and otic vesicle, although their levels and timing of expression differed markedly. LjTbx1/10A was highly expressed in the mesodermal core of pharyngeal arches and the adjacent endoderm throughout pharyngeal arch development, whereas LjTbx1/10B expression was only transiently upregulated in forming pharyngeal pouches. LjTbx1/10A transcripts first appeared at stage 25 in otic vesicles, whereas LjTbx1/10B transcripts could already be detected in the developing otic placode at stage 20. These results suggest that lamprey LjTbx1/10A and LjTbx1/10B may play distinct roles in the patterning and development of the pharyngeal apparatus. It appears that lamprey Tbx1/10 genes have undergone subfunctionalization independent from gnathostomes, with regard to both regulation and function.
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Acknowledgments
We thank Ebetsu fishery cooperative for providing adult male and female lampreys. E. T. was supported by the JSPS Postdoctoral Fellowship for Foreign Researchers (PE05004). This work was partly supported by a Grant-in-Aid for Young Scientists Category A from the Ministry of Education, Science, Sports and Culture of Japan, a Tokyo Tech Award for Challenging Research and a research grant to M. T. from the Hayashi Memorial Foundation of Female Natural Scientists.
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Communicated by M. Hammerschmidt
Tiecke and Matsuura contributed equally to this work.
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Tiecke, E., Matsuura, M., Kokubo, N. et al. Identification and developmental expression of two Tbx1/10-related genes in the agnathan Lethenteron japonicum . Dev Genes Evol 217, 691–697 (2007). https://doi.org/10.1007/s00427-007-0181-0
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DOI: https://doi.org/10.1007/s00427-007-0181-0