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hedgehog is a segment polarity gene in a crustacean and a chelicerate

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Abstract

The evolution of arthropod segmentation has been studied by comparing expression patterns of pair-rule and segment polarity genes in various species. In Drosophila, the formation and maintenance of the parasegmental boundaries depend on the interactions between the wingless (wg), engrailed (en) and hedgehog (hh) genes. Until now, the expression pattern of hh has not been analysed to such a great extent as en or wg. We report the cloning and expression analysis of hh genes from Euscorpius flavicaudis, a chelicerate, and Artemia franciscana, a branchiopod crustacean. Our data provide evidence that hh, being expressed in the posterior part of every segment, is a segment polarity gene in both organisms. Additional hh expression sites were observed in the rostrum and appendages of Euscorpius and in the gut of Artemia. From the available data on hh expression in various bilaterians, we review the various hypotheses on the evolution of hh function and we suggest an ancestral role of hh in proctodeum specification and gut formation.

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Acknowledgements

The authors would like to thank Roland Stockmann (University Paris 6) for his kind help in collecting animals and for discussions about the development of Euscorpius, Maryline Blin for her molecular expertise and Nicolas Rabet for help with SEM. The advised comments of Michael Manuel helped improve the manuscript. We acknowledge Diethard Tautz for his helpful remark on the difference between chelicerates and pan-crustaceans. F.S. is the recipient of a Ph.D. fellowship from the Ministère de la Recherche et de la Technologie.

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  1. Evolution et Développement UMR 7138—Systématique, Adaptation et Evolution, Université Pierre et Marie Curie (Paris 6), 9 quai Saint-Bernard, Case 5, 75252, Paris Cedex 05, France

    Franck Simonnet & Eric Quéinnec

  2. Développement et Evolution UMR 7622—Biologie du Développement, Université Pierre et Marie Curie (Paris 6), 9 quai Saint-Bernard, Case 24, 75252, Paris Cedex 05, France

    Jean Deutsch

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  1. Franck Simonnet
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Correspondence to Eric Quéinnec.

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Simonnet, F., Deutsch, J. & Quéinnec, E. hedgehog is a segment polarity gene in a crustacean and a chelicerate. Dev Genes Evol 214, 537–545 (2004). https://doi.org/10.1007/s00427-004-0435-z

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