Abstract
xFoxJ1 and xFoxK1, two novel members of the fork head/winged helix family of transcription factors in Xenopus, have been identified by sequence homology to their mammalian orthologues. We here report the complete amino acid sequences of xFoxJ1, its pseudo-allelic version xFoxJ1′, and of xFoxK1, the latter also comprising a fork-head-associated (FHA) domain. We have further analysed the temporal and spatial expression of both genes. xFoxJ1 transcripts were detected during gastrulation in the dorsal blastopore lip and in the animal half. A predominant feature of xFoxJ1 expression until the tailbud stage is its spotty pattern within the epidermis. xFoxK1 is maternally expressed and localized within the animal half during early cleavage stages. Zygotic transcription starts during neurulation and is initially restricted to neuroectoderm. As development proceeds, xFoxK1 is expressed in ectoderm- and mesoderm-derived tissues, like the branchial arches, brain, eye, otic vesicle, pronephros, somites and abdominal muscle precursors.
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Bassel-Duby R, Hernandez MD, Yang Q, Rochelle JM, Seldin MF, Williams RS (1994) Myocyte nuclear factor, a novel winged-helix transcription factor under both developmental and neural regulation in striated myocytes. Mol Cell Biol 14:4596–4605
Brody SL, Yan XH, Wuerffel MK, Song SK, Shapiro SD (2000) Ciliogenesis and left-right axis defects in forkhead factor HFH-4-null mice. Am J Respir Cell Mol Biol 23:45–51
Clevidence DE, Overdier DG, Tao W, Qian X, Pani L, Lai E, Costa RH (1993) Identification of nine tissue-specific transcription factors of the hepatocyte nuclear factor 3/forkhead DNA-binding-domain family. Proc Natl Acad Sci USA 90:3948–3952
Dirksen ML, Jamrich M (1995) Differential expression of forkhead genes during early Xenopus and zebrafish development. Dev Genet 17:107–116
Hackett BP, Brody SL, Liang M, Zeitz ID, Bruns LA, Gitlin JD (1995) Primary structure of hepatocyte nuclear factor/forkhead homologue 4 and characterization of gene expression in the developing respiratory and reproductive epithelium. Proc Natl Acad Sci USA 92:4249–4253
Harland RM (1991) In situ hybridization: an improved whole-mount method for Xenopus embryos. Methods Cell Biol 36:685–695
Hawke TJ, Jiang N, Garry D (2003) Absence of p21CIP rescues myogenic progenitor cell proliferative and regenerative capacity in Foxk1 null mice. J Biol Chem 278:4015–4020
Kaestner KH, Knöchel W, Martinez DE (2000) Unified nomenclature for the winged helix/forkhead transcription factors. Genes Dev 14:142–146
Lee G, Ding Z, Walker JC, Van Doren SR (2003) NMR structure of the forkhead-associated domain from the Arabidopsis receptor kinase-associated protein phosphatase. Proc Natl Acad Sci USA 100:11261–11266
Li C, Lai CF, Sigman DS, Gaynor RB (1991) Cloning of a cellular factor, interleukin binding factor, that binds to NFAT-like motifs in the human immunodeficiency virus long terminal repeat. Proc Natl Acad Sci USA 88:7739–7743
Li J, Smith GP, Walker JC (1999) Kinase interaction domain of kinase-associated protein phosphatase, a phosphoprotein-binding domain. Proc Natl Acad Sci USA 96:7821–7826
Murphy DB, Seemann S, Wiese S, Kirschner R, Grzeschik KH, Thies U (1997) The human hepatocyte nuclear factor 3/fork head gene FKHL13: genomic structure and pattern of expression. Genomics 40:462–469
Nieuwkoop PD, Faber J (1967) Normal table of Xenopus laevis (Daudin), 2nd edn. Elsevier/North Holland, Amsterdam
Nirula A, Moore DJ, Gaynor RB (1997) Constitutive binding of the transcription factor interleukin-2 (IL-2) enhancer binding factor to the IL-2 promoter. J Biol Chem 272:7736–7745
Pelletier GJ, Brody SL, Liapis H, White RA, Hackett BP (1998) A human forkhead/winged-helix transcription factor expressed in developing pulmonary and renal epithelium. Am J Physiol 274:L351–L359
Satou Y, Yamada L, Mochizuki Y, Takatori N, Kawashima T, Sasaki A, Hamaguchi M, Awazu S, Yagi K, Sasakura Y, Nakayama A, Ishikawa H, Inaba K, Satoh N (2002) A cDNA resource from the basal chordate Ciona intestinalis. Genesis 33:153–154
Acknowledgements
We thank G. Schlosser and K. Astrahantseff for helpful discussions of the manuscript and K. Botzenhart and C. Donow for skilful technical assistance. This investigation was aided by grants from the Deutsche Forschungsgemeinschaft (SFB 497/A 3) and by Fonds der Chemischen Industrie.
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Pohl, B.S., Knöchel, W. Isolation and developmental expression of Xenopus FoxJ1 and FoxK1. Dev Genes Evol 214, 200–205 (2004). https://doi.org/10.1007/s00427-004-0391-7
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DOI: https://doi.org/10.1007/s00427-004-0391-7