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A Dickkopf-3-related gene is expressed in differentiating nematocytes in the basal metazoan Hydra

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Abstract

In vertebrate development the Dickkopf protein family carries out multiple functions and is represented by at least four different genes with distinct biological activities. In invertebrates such as Drosophila and Caenorhabditis, Dickkopf genes have so far not been identified. Here we describe the identification and characterization of a Dickkopf gene with a deduced amino acid sequence closely related to that of chicken Dkk-3 in the basal metazoan Hydra. HyDkk-3 appears to be the only Dickkopf gene in Hydra. The gene is expressed in the gastric region in nematocytes at a late differentiation stage. In silico searches of EST and genome databases indicated the absence of Dkk genes from the protostomes Drosophila and Caenorhabditis, whereas within the deuterostomes, a Dkk-3 gene could be identified in the genome of the urochordate Ciona intestinalis. The results indicate that at an early stage of evolution of multicellularity Dickkopf proteins have already played important roles as developmental signals. They also suggest that vertebrate Dkk-1, 2 and 4 may have originated from a common ancestor gene of Dkk-3.

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Acknowledgements

We thank Konstantin Khalturin for help with the in silico analysis and him and Ulrich Technau for discussion and critically reading the manuscript. Supported by the Deutsche Forschungsgemeinschaft.

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Authors and Affiliations

  1. Zoological Institute, Christian-Albrechts-University Kiel, Olshausenstrasse 40, 24098, Kiel, Germany

    Henning Fedders, René Augustin & Thomas C. G. Bosch

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  1. Henning Fedders
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  2. René Augustin
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  3. Thomas C. G. Bosch
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Correspondence to Thomas C. G. Bosch.

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H. Fedders and R. Augustin contributed equally to this work

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Fedders, H., Augustin, R. & Bosch, T.C.G. A Dickkopf-3-related gene is expressed in differentiating nematocytes in the basal metazoan Hydra . Dev Genes Evol 214, 72–80 (2004). https://doi.org/10.1007/s00427-003-0378-9

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