Abstract
Here I present gene expression data that implicate a Notch-mediated lateral inhibition process in the spatial organization of butterfly wing scales. During early pupal development the receptor molecule Notch is expressed in a grid-like pattern in the wing epithelium, resulting in parallel rows of uniformly spaced cells with low Notch expression. Previous work has shown that these low-Notch cells express a homolog of the achaete-scute proneural transcription factors and develop into scales. All of these observations are consistent with the Drosophila model of Notch-mediated bristle determination and support the hypothesis that bristles and scales share an underlying patterning mechanism.
References
Artavanis-Tsakonas S, Rand MD, Lake RJ (1999) Notch signaling: cell fate control and signal integration in development. Science 284:770–776
Brunetti CR, Selegue JE, Monteiro A, French V, Brakefield PM, Carroll SB (2001) The generation and diversification of butterfly eyespot color patterns. Curr Biol 11:1578–1585
Calleja M, Renaud O, Usui K, Pistillo D, Morata G, Simpson P (2002) How to pattern an epithelium: lessons from achaete-scute regulation on the notum of Drosophila. Gene 292:1–12
Fehon RG, Kooh PJ, Rebay I, Regan CL, Xu T, Muskavitch MAT, Artavanis-Tsakonas S (1990) Molecular interactions between the protein products of the neurogenic loci Notch and Delta, 2 EGF-homologous genes in Drosophila. Cell 61:523–534
Galant R, Skeath JB, Paddock S, Lewis DL, Carroll SB (1998) Expression pattern of a butterfly achaete-scute homolog reveals the homology of butterfly wing scales and insect sensory bristles. Curr Biol 8:807–813
Honda H, Tanemura M, Yoshida A (2000) Differentiation of wing epidermal scale cells in a butterfly under the lateral inhibition model—appearance of large cells in a polygonal pattern. Acta Biotheoret 48:121–136
Koch PB, Merk R, Reinhardt R, Weber P (2003) Localization of ecdysone receptor protein during colour pattern formation in wings of the butterfly Precis coenia (Lepidoptera: Nymphalidae) and co-expression with Distal-less protein. Dev Genes Evol 212:571–584
Mayer AG (1896) The development of the wing scales and their pigment in butterflies and moths. Bull Mus Comp Zool 29:209–236
Nardi JB, Magee-Adams SM (1986) Formation of scale spacing patterns in a moth wing. 1. Epithelial feet may mediate cell rearrangement. Dev Biol 116:278–290
Nijhout HF (1991) The development and evolution of butterfly wing patterns. Smithsonian Institution Press, Washington, D.C.
Overton J (1966) Microtubules and microfibrils in morphogenesis of the scale cells of Ephestia kühniella. J Cell Biol 29:293–305
Panganiban G, Sebring A, Nagy L, Carroll S (1995) The development of crustacean limbs and the evolution of arthropods. Science 270:1363–1366
Scoble MJ (1992) The Lepidoptera: form, function, and diversity. Oxford University Press, Oxford
Simpson P, Woehl R, Usui K (1999) The development and evolution of bristle patterns in Diptera. Development 126:1349–1364
Wigglesworth V (1972) The principles of insect physiology. Methuen, New York
Yoshida A, Aoki K (1989) Scale arrangement pattern in a lepidopteran wing. 1. Periodic cellular pattern in the pupal wing of Pieris rapae. Dev Growth Differ 31:601–609
Acknowledgements
I thank Lisa M. Nagy and the referees for comments on the manuscript, Sean B. Carroll for the Distal-less antibody, Developmental Studies Hybridoma Bank for the Notch antibody, and W. Owen McMillan for H. erato. This work was supported by National Science Foundation grant DEB 0209441.
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Reed, R.D. Evidence for Notch-mediated lateral inhibition in organizing butterfly wing scales. Dev Genes Evol 214, 43–46 (2004). https://doi.org/10.1007/s00427-003-0366-0
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DOI: https://doi.org/10.1007/s00427-003-0366-0