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Inhibition of metamorphosis by RFamide neuropeptides in planula larvae of Hydractinia echinata

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Abstract

The primitive nervous system in planula larvae of Hydractinia echinata (Cnidaria) has sensory neurons containing LWamide or RFamide neuropeptides. LWamides have been shown to induce metamorphosis of planula larvae into adult polyps. We report here that RFamides act antagonistically to LWamides. RFamides inhibit metamorphosis when applied to planula larvae during metamorphosis induction by treatment with LWamides (or other inducing agents such as CsCl ions, diacylglycerol and bacterial inducers). Our results show further that RFamides act downstream of LWamide release, presumably directly on target cells mediating metamorphosis. These observations support a model in which metamorphosis in H. echinata is regulated by sensory neurons secreting LWamides and RFamides in response to environmental cues.

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Acknowledgements

We thank Drs. Y. Muneoka and T. Takahashi for a gift of hydra peptides, Dr. T. Leitz for a gift of A. espejiana culture, Drs. T. Fujisawa and M. Hatta for valuable discussions, and Ms. A. Zychlinski for enthusiastic technical assistance. T.S. was a recipient of the Alexander von Humboldt-Foundation Research Award, which enabled him to initiate this project in the laboratory of C.N.D. This work was supported in part by grants from Sumitomo Foundation and Yamada Science Foundation to T.S. and by grants from the German Science Foundation (DFG) to C.N.D.

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Author notes

  1. Yuki Katsukura

    Present address: Zoologisches Institut der Universitaet Heidelberg, Im Neuenheimer Feld 230, 69120, Heidelberg, Germany

  2. Tsutomu Sugiyama

    Present address: , Kamizawa 952-40, Kan-nami-Cho, Tagata-Gun, 419-0122, Shizuoka-Ken, Japan

Authors and Affiliations

  1. Ishinomaki Senshu University, 986-8580, Ishinomaki, Japan

    Yuki Katsukura & Tsutomu Sugiyama

  2. Department Biologie II, Ludwig-Maximilians-Universität München, 80333, Munich, Germany

    Charles N. David

  3. Zoological Institute, Department of Cell Biology, University of Copenhagen, 2100, Copenhagen, Denmark

    Cornelis J. P. Grimmelikhuijzen

Authors
  1. Yuki Katsukura
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  2. Charles N. David
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  3. Cornelis J. P. Grimmelikhuijzen
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  4. Tsutomu Sugiyama
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Correspondence to Yuki Katsukura.

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Katsukura, Y., David, C.N., Grimmelikhuijzen, C.J.P. et al. Inhibition of metamorphosis by RFamide neuropeptides in planula larvae of Hydractinia echinata . Dev Genes Evol 213, 579–586 (2003). https://doi.org/10.1007/s00427-003-0361-5

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