Identification and expression of HySmad1, a member of the R-Smad family of TGFβ signal transducers, in the diploblastic metazoan Hydra

Abstract.

Members of the Smad family of TGFβ signal transducers are important regulators of proliferation and cell fate during axis formation, organogenesis, and tumorigenesis. A canonical TGFβ/Smad signaling pathway is conserved in nematodes, insects, and vertebrates. However, its evolutionary origin before the divergence of protostomes and deuterostomes is unclear. Here, we present the cloning and expression of a highly conserved orthologue of receptor-activated Smads in Hydra, which represents clear evidence of the presence of TGFβ signaling in the ancient phylum Cnidaria. HySmad1 is expressed rather uniformly during asexual reproduction and regeneration, and is transcriptionally upregulated during oocyte development. This suggests that multiple functions of TGFβ/Smad signaling might be conserved between diploblastic and triploblastic metazoans.