Abstract
Main conclusion
Through combined analysis of the transcriptome and targeted metabolome of lily bulbs, the possible molecular mechanism of dormancy release was revealed.
Abstract
Regulation of bulb dormancy is critical for ensuring annual production and high-quality cultivation. The application of low temperatures is the most effective method for breaking bulb dormancy, but the molecular mechanism underlying this response is unclear. Herein, targeted metabolome and transcriptome analyses were performed on Lilium davidii var. unicolor bulbs stored for 0, 50, and 100 days at 4 °C. Dormancy release mainly depended on the accumulation of gibberellins GA4 and GA7, which are synthesized by the non-13-hydroxylation pathway, rather than GA3, and ABA was degraded in the process. The contents of nonbioactive GA9, GA15, and GA24, the precursors of GA4 synthesis, increased with bulb dormancy release. Altogether, 113,252 unique transcripts were de novo assembled through high-throughput transcriptome sequences, and 639 genes were continuously differentially expressed. Energy sources during carbohydrate metabolism mainly depend on glycolysis and the pentose phosphate pathway. Screening of transcription factor families involved in bulb dormancy release showed that MYB, WRKY, NAC, and TCP members were significantly correlated with the targeted metabolome. Coexpression analysis further confirmed that ABI5, PYL8, PYL4, and PP2C, which are vital ABA signaling elements, regulated GA3ox and GA20ox in the GA4 biosynthesis pathway, and XERICO may be involved in the regulation of ABA and GA4 signaling through the ubiquitination pathway. WRKY32, WRKY71, DAM14, NAC8, ICE1, bHLH93, and TCP15 also participated in the ABA/GA4 regulatory network, and ICE1 may be the key factor linking temperature signals and hormone metabolism. These results will help to reveal the bulb dormancy molecular mechanism and develop new strategies for high-quality bulb production.
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Data availability
The datasets generated during the current study are not publicly available as lily does not yet have publicly available genomic data, all of our subsequent studies rely on existing data but are available from the corresponding author on reasonable request.
Code availability
Not applicable.
Abbreviations
- DEGs:
-
Differentially expressed genes
- DD:
-
Deep dormancy
- FDR:
-
Full dormancy release
- GAs:
-
Gibberellins
- JA:
-
Jasmonic acid
- PDR:
-
Partial dormancy release
- PPP:
-
Pentose phosphate pathway
- SA:
-
Salicylic acid
- TCA:
-
Tricarboxylic acid
- TF:
-
Transcription factor
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Funding
This work was supported by the National Key R&D Program of China “Post-harvest quality maintenance mechanism and regulation of key flowers” (2018YFD1000407, Ministry of Science and Technology of the People's Republic of China).
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Fan, X., Yang, Y., Li, M. et al. Transcriptomics and targeted metabolomics reveal the regulatory network of Lilium davidii var. unicolor during bulb dormancy release. Planta 254, 59 (2021). https://doi.org/10.1007/s00425-021-03672-7
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DOI: https://doi.org/10.1007/s00425-021-03672-7