Abstract
Main conclusion
Bioinformatic, molecular, and biochemical analysis were performed to get more insight into the regulatory mechanism by which TmHKT1;4-A2 is regulated.
Abstract
HKT transporters from different plant species have been shown to play important role in plant response to salt. In previous work, TmHKT1;4-A2 gene from Triticum monococcum has been characterized as a major gene for Nax1 QTL (Tounsi et al. Plant Cell Physiol 57:2047–2057, 2016). So far, little is known about its regulatory mechanism. In this study, the promoter region of TmHKT1;4-A2 (1400 bp) was isolated and considered as the full-length promoter (PA2-1400). In silico analysis revealed the presence of important cis-acting elements related to abiotic stresses and phytohormones. Interestingly, our real-time RT-PCR analysis provided evidence that TmHKT1;4-A2 is regulated not only by salt stress but also by osmotic, heavy metal, oxidative, and hormones stresses. In transgenic Arabidopsis plants, TmHKT1;4-A2 is strongly active in vascular tissues of roots and leaves. Through 5′-end deletion analysis, we showed that PA2-1400 promoter is able to drive strong GUS activity under normal conditions and in response to different stresses compared to PA2-824 and PA2-366 promoters. These findings provide new information on the regulatory mechanism of TmHKT1;4-A2 and shed more light on its role under different stresses.
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Abbreviations
- CAMTA:
-
Calmodulin-binding transcription activator
- HKT:
-
High-affinity potassium transporter
- QTL:
-
Quantitative trait locus
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Acknowledgements
We are thankful to ICARDA for the provision of Seeds of Triticum monococcum (cv. Turkey).
Funding
This study was supported by a grant from the Ministry of Higher Education and Scientific Research, Tunisia, and by a research grant from research office, United Arab Emirates University (Grant No. 31F096 to KM).
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Tounsi, S., Saïdi, M.N., Abdelhedi, R. et al. Functional analysis of TmHKT1;4-A2 promoter through deletion analysis provides new insight into the regulatory mechanism underlying abiotic stress adaptation. Planta 253, 18 (2021). https://doi.org/10.1007/s00425-020-03533-9
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DOI: https://doi.org/10.1007/s00425-020-03533-9