Abstract
Main conclusions
The 4-coumarate-CoA ligases (4CL) contribute in channelizing flux of different phenylpropanoid biosynthetic pathways. Expression of 4CL is optimized at developmental stages and in response to environmental triggers such as biotic and abiotic stresses. The enzyme is valuable in metabolic pathway engineering for curcuminoids, resveratrol, biofuel production and nutritional improvement. Vigorous analysis of regulation at functional and expression level is obligatory to attain efficient commercial production of candidate metabolites using 4CL.
Phenylpropanoid pathway provides precursors for numerous secondary metabolites in plants. In this pathway, 4-coumarate-CoA ligase (EC 6.2.1.12, 4CL) is the main branch point enzyme which generates activated thioesters. Being the last enzyme of three shared common steps in general phenylpropanoid pathway, it contributes to channelize precursors for different phenylpropanoids. In plants, 4CL enzymes are present in multiple isoforms and encoded by small gene family. It belongs to adenylate-forming enzyme family and catalyzes the reaction that converts hydroxy or methoxy cinnamic acid derivatives to corresponding thioesters. These thioesters are further utilized for biosynthesis of phenylpropanoids, which are known for having numerous nutritional and medicinal applications. In addition, the 4CL enzymes have been characterized from various plants for their role in plant physiology or in biotic and abiotic stresses. Furthermore, specific isoforms are differentially regulated upon exposure to diverse stimuli leading to flux diversion toward the particular metabolite biosynthesis. Evolutionary studies showed that 4CL separately evolved after monocot and dicot segregation. Here, we provide a comprehensive review on 4CL, which includes evolution, function, gene/protein structure, role in metabolite biosynthesis and cellular partition, and their regulation. Based on the available data, we have explored the scope for pathway engineering by utilizing 4CL enzymes.
Similar content being viewed by others
Abbreviations
- 4CL:
-
4-coumarate:CoA ligase
- C3H:
-
p-coumarate 3-hydroxylase
- C4H:
-
Cinnamate 4-hydroxylase
- CAD:
-
Cinnamyl alcohol dehydrogenase
- CCoAOMT:
-
Caffeoyl-CoA O-methyltransferase
- PAL:
-
Phenylalanine ammonia lyase
- CVOMT:
-
Chavicol O-methyltransferase
- COMT:
-
Caffeic acid O-methyltransferase
- EOMT:
-
Eugenol O-methyltransferase
- F5H:
-
Ferulate 5-hydroxylase
- CAD:
-
Cinnamyl alcohol dehydrogenase
- CHS:
-
Chalcone synthase
- ANS, F5H:
-
Ferulate 5-hydroxylase
- HCT:
-
p-hydroxycinnamoyl-CoA:quinate shikimate p-hydroxycinnamoyltransferase
- DFR:
-
Dihydroflavonol 4-reductase
References
Allina SM, Pri-Hadash A, Theilmann DA et al (1998) 4-Coumarate-coenzyme A ligase in hybrid poplar properties of native enzyme, cDNA cloning, and analysis of recombinant enzyme. Plant Physiol 116:743–754. https://doi.org/10.1104/pp.116.2.743
Amalraj A, Pius A, Gopi S et al (2017) Biological activities of curcuminoids, other biomolecules from turmeric and their derivatives—a review. J Tradit Complement Med 7(2):205–233. https://doi.org/10.1016/j.jtcme.2016.05.005
An CH, Lee KW, Lee SH et al (2015) Heterologous expression of IbMYB1a by different promoters exhibits different patterns of anthocyanin accumulation in tobacco. Plant Physiol Biochem 89:1–10. https://doi.org/10.1016/j.plaphy.2015.02.002
Anand A, Jayaramaiah RH, Beedkar SD et al (2016) Comparative functional characterization of eugenol synthase from four different Ocimum species: implications on eugenol accumulation. Biochim Biophys Acta Proteins Proteom 1864(11):1539–1547. https://doi.org/10.1016/j.bbapap.2016.08.004
Baldi P, Moser M, Brilli M et al (2017) Fine-tuning of the flavonoid and monolignol pathways during apple early fruit development. Planta 245:1021–1035. https://doi.org/10.1007/s00425-017-2660-5
Becker-Andre M, Schulze-Lefert P, Hahlbrock K (1991) Structural comparison, modes of expression, and putative cis-acting elements of the two 4-Coumarate: CoA ligase genes in potato. J Biol Chem 266:8551–8559
Bennett RN, Wallsgrove RM (1994) Secondary metabolites in plant defence mechanisms. New Phytol 127:617–633. https://doi.org/10.1111/j.1469-8137.1994.tb02968.x
Blanco-Ulate B, Hoper H, Figueroa-Balderas R et al (2017) Red blotch disease alters grape berry development and metabolism by interfering with the transcriptional and hormonal regulation of ripening. J Exp Bot 68(5):1225–1238. https://doi.org/10.1093/jxb/erw506
Bolton MD (2009) Primary metabolism and plant defense-fuel for the fire. Mol Plant Microbe Interact 22(5):487–497. https://doi.org/10.1094/MPMI-22-5-0487
Burbulis IE, Winkel-Shirley B (1999) Interactions among enzymes of the Arabidopsis flavonoid biosynthetic pathway. Proc Natl Acad Sci USA 96(22):12929–12934. https://doi.org/10.1073/pnas.96.22.12929
Cao Y, Han Y, Li D et al (2016) Systematic analysis of the 4-Coumarate: coenzyme A ligase (4CL) related genes and expression profiling during Fruit development in the Chinese Pear. Genes 7(10):89. https://doi.org/10.3390/genes7100089
Chandran D, Sharapova N, Ivashuta S et al (2008) Transcriptome profiling identified novel genes associated with aluminum toxicity, resistance and tolerance in Medicago truncatula. Planta 228:151–166. https://doi.org/10.1007/s00425-008-0726-0
Chemler JA, Koffas MA (2008) Metabolic engineering for plant natural product biosynthesis in microbes. Curr Opin Biotechnol 19:597–605. https://doi.org/10.1016/j.copbio.2008.10.011
Chen HC, Song J, Wang JP et al (2014a) Systems biology of lignin biosynthesis in Populus trichocarpa: heteromeric 4-coumaric acid: Coenzyme A ligase protein complex formation, regulation, and numerical modeling. Plant Cell 26:876–893. https://doi.org/10.1105/tpc.113.119685
Chen HY, Babst BA, Nyamdari B et al (2014b) Ectopic expression of a Loblolly Pine class II 4-Coumarate:CoA ligase alters soluble phenylpropanoid metabolism but not lignin biosynthesis in Populus. Plant Cell Physiol 55(9):1669–1678. https://doi.org/10.1093/pcp/pcu098
Choudhary EK, Choi B, Cho BK et al (2013) Regulation of 4CL, encoding 4-coumarate: coenzyme A ligase, expression in kenaf under diverse stress conditions. Plant Omics J. 6(4):254–262
Citti C, Battisti UM, Braghiroli D et al (2017) A metabolomic approach applied to a liquid chromatography coupled to high-resolution tandem mass spectrometry method (HPLC–ESI–HRMS/MS): towards the comprehensive evaluation of the chemical composition of cannabis medicinal extracts. Phytochem Anal 29:144–155. https://doi.org/10.1002/pca.2722
Costa MA, Bedger DL, Moinuddin SGA et al (2005) Characterization in vitro and in vivo of the putative multigene 4-coumarate: CoA ligase network in Arabidopsis: syringyl lignin and sinapate/sinapyl alcohol derivative formation. Phytochemistry 66:2072–2091. https://doi.org/10.1016/j.phytochem.2005.06.022
Cukovic D, Ehlting J, Vanziffle JA (2001) Structure and evolution of 4-Coumarate: coenzyme A ligase (4CL) gene families. Biol Chem 382:645–654. https://doi.org/10.1515/BC.2001.076
Dixon RA, Paiva NL (1995) Stress-induced phenylpropanoid metabolism Plant Cell 7:1085–1097. https://doi.org/10.1105/tpc.7.7.1085
Douglas CJ (1996) Phenylpropanoid metabolism and lignin biosynthesis: from weeds to trees. Trends Plant Sci 1(6):171–178. https://doi.org/10.1016/1360-1385(96)10019-4
Douglas C, Hoffmann H, Wolfgang S et al (1987) Structure and elicitor or uv-light-stimulated expression of two 4-coumarate-CoA ligase genes in parsley. EMBO J 6(5):1189–1195
Ehlting J, Buttner D, Wang Q et al (1999) Three 4-coumarate:coenzyme A ligases in Arabidopsis thaliana represent two evolutionarily divergent classes in angiosperms. Plant J 19:9–20. https://doi.org/10.1046/j.1365-313X.1999.00491.x
Ehlting J, Shin JJK, Douglas CJ (2001) Identification of 4-coumarate:coenzyme A ligase (4CL) substrate recognition domains. Plant J 25:455–465. https://doi.org/10.1046/j.1365-313X.2001.01122.x
Ferrieri AP, Arce CCM, Machado RAR et al (2015) A Nicotiana attenuata cell wall invertase inhibitor (NaCWII) reduces growth and increases secondary metabolite biosynthesis in herbivore-attacked plants. New Phytol 208:519–530. https://doi.org/10.1111/nph.13475
Floros DJ, Petras D, Kapono CA et al (2017) Mass spectrometry based molecular 3D-cartography of plant metabolites. Front Plant Sci 8:429. https://doi.org/10.3389/fpls.2017.00429
Fraser CM, Chapple C (2011) The phenylpropanoid pathway in Arabidopsis. The Arabidopsis Book/American Society of Plant Biologists 9:e0152. https://doi.org/10.1199/tab.0152
Freund DM, Hegeman AD (2017) Recent advances in stable isotope-enabled mass spectrometry-based plant metabolomics. Curr Opin Biotechnol 43:41–48. https://doi.org/10.1016/j.copbio.2016.08.002
Fritzemeier KH, Cretin C, Kombrink E et al (1987) Transient induction of phenylalanine ammonia-lyase and 4-Coumarate:CoA ligase mRNAs in Potato leaves infected with virulent or avirulent races of Phytophthora infestans. Plant Physiol 85:34–41. https://doi.org/10.1104/pp.85.1.34
Fujino N, Tenma N, Waki T et al (2018) Physical interactions among flavonoid enzymes in snapdragon and torenia reveal the diversity in the flavonoid metabolon organization of different plant species. Plant J 94(2):372–392. https://doi.org/10.1111/tpj.13864
Fukushima A, Kusano M, Redestig H et al (2009) Integrated omics approaches in plant systems biology. Curr Opin Chem Biol 13:532–538. https://doi.org/10.1016/j.cbpa.2009.09.022
Fulda M, Heinz E, Wolter FP (1994) The fadD gene of Escherichia coli K12 is located close to rnd at 39.6 min of the chromosomal map and is a new member of the AMP-binding protein family. Mol Gen Genet 242:241–249. https://doi.org/10.1007/BF00280412
Gaid MM, Scharnhop H, Ramadan H et al (2011) 4-Coumarate:CoA ligase family members from elicitor-treated Sorbus aucuparia cell cultures. J Plant Physiol 168:944–951. https://doi.org/10.1016/j.jplph.2010.11.021
Gao S, Yu HN, Xu RX et al (2015) Cloning and functional characterization of a 4-coumarate CoA ligase from liverwort Plagiochasma appendiculatum. Phytochemistry 111:48–58. https://doi.org/10.1016/j.phytochem.2014.12.017
Giovinazzo G, Ingrosso I, Paradiso A et al (2012) Resveratrol biosynthesis: plant metabolic engineering for nutritional improvement of food. Plant Foods Hum Nutr 67:191–199. https://doi.org/10.1007/s11130-012-0299-8
Goufo P, Moutinho-Pereira JM, Jorge TF et al (2017) Cowpea (Vigna unguiculata L. Walp.) metabolomics: osmoprotection as a physiological strategy for drought stress resistance and improved yield. Front. Plant Sci 8:586. https://doi.org/10.3389/fpls.2017.00586
Gui J, Shen J, Li L (2011) Functional characterization of evolutionarily divergent 4-Coumarate: coenzyme A ligases in Rice. Plant Physiol 157:574–586. https://doi.org/10.1104/pp.111.178301
Hahlbrock K, Scheel D (1989) Physiology and molecular biology of phenylpropanoid metabolism. Ann Rev Plant Physiol 40:347–369
Hamada K, Nishida T, Yamauchi K et al (2004) 4-Coumarate: coenzyme A ligase in black locust (Robinia pseudoacacia) catalyses the conversion of sinapate to sinapoyl-CoA. J Plant Res 117:303–310. https://doi.org/10.1007/s10265-004-0159-1
Herrmann KM, Weaver LM (1999) The shikimate pathway. Annu Rev Plant Physiol Plant Mol Biol 50:473–503. https://doi.org/10.1146/annurev.arplant.50.1.473
Holton TA, Cornish EC (1995) Genetics and biochemistry of anthocyanin biosynthesis. Plant Cell 7:1071–1083. https://doi.org/10.1105/tpc.7.7.1071
Hrazdina G, Wagner GJ (1985) Metabolic pathways as enzyme complexes: evidence 641 for the synthesis of phenylpropanoids and flavonoids on membrane associated enzyme 642 complexes. Arch Biochem Biophys 237:88–100
Hu W, Kawaoka A, Tsai CJ et al (1998) Compartmentalized expression of two structurally and functionally distinct 4-coumarate: coenzyme A ligase (4CL) genes in Aspen (Populus tremuloides). Proc Natl Acad Sci USA 95:5407–5412. https://doi.org/10.1073/pnas.0307307101
Hu WJ, Harding SA, Lung J et al (1999) Repression of lignin biosynthesis promotes cellulose accumulation and growth in transgenic trees. Nat Biotechnol 17:808–812. https://doi.org/10.1038/11758
Hu Y, Gai Y, Yin L et al (2010) Crystal structures of a Populus tomentosa 4-coumarate: CoA ligase shed light on its enzymatic mechanisms. Plant Cell 22:3093–3104. https://doi.org/10.1105/tpc.109.072652
Hue HTT, Ha DTT, Hai NV et al (2016) Isolation and characterization of the 4-coumarate:coenzyme A ligase (4CL1) promoter from Eucalyptus camaldulensis. Physiol Mol Biol Plants 22(3):399–405. https://doi.org/10.1007/s12298-016-0369-8
Jin H, Cominell E, Bailey P et al (2000) Transcriptional repression by AtMYB4 controls production of UV-protecting sunscreens in Arabidopsis. EMBO J 19(22):6150–6161. https://doi.org/10.1093/emboj/19.22.6150
Jung JH, Kannan B, Dermawan H (2016) Precision breeding for RNAi suppression of a major 4-coumarate:coenzyme A ligase gene improve cell wall saccharification from field grown sugarcane. Plant Mol Biol 92:505–517. https://doi.org/10.1007/s11103-016-0527-y
Katsuyama Y, Matsuzawa M, Funa N et al (2008) Production of curcuminoids by Escherichia coli carrying an artificial biosynthesis pathway. Microbiology 154:2620–2628. https://doi.org/10.1099/mic.0.2008/018721-0
Kim YB, Kim JK, Uddin MR et al (2013) Metabolomics analysis and biosynthesis of Rosmarinic acid in Agastache rugosa Kuntze treated with Methyl Jasmonate. PLoS One 8(5):e64199. https://doi.org/10.1371/journal.pone.0064199
Korkina LG (2007) Phenylpropanoids as naturally occurring antioxidants: from plant defense to human health. Cell Mol Biol 53:15–25. https://doi.org/10.1170/T772
Kueger S, Steinhauser D, Willmitzer L et al (2012) High-resolution plant metabolomics: from mass spectral features to metabolites and from whole-cell analysis to subcellular metabolite distributions. Plant J 70:39–50. https://doi.org/10.1111/j.1365-313X.2012.04902.x
Lee D, Douglas CJ (1996) Two divergent members of a tobacco 4-Coumarate: coenzyme A ligase (4CL) gene family (cDNA structure, gene inheritance and expression, and properties of recombinant proteins). Plant Physiol 112:193–205. https://doi.org/10.1104/pp.112.1.193
Li Z, Nair SK (2015) Structural basis for specificity and flexibility in a plant 4-coumarate:CoA ligase. Structure 23:2032–2042. https://doi.org/10.1016/j.str.2015.08.012
Li ZB, Li CF, Li J et al (2014) Molecular cloning and functional characterization of two divergent 4-Coumarate: coenzyme A ligases from Kudzu (Pueraria lobata). Biol Pharm Bull 37(1):113–122. https://doi.org/10.1248/bpb.b13-00633
Mandoulakani BA, Eyvazpour E, Ghadimzadeh M (2017) The effect of drought stress on the expression of key genes involved in the biosynthesis of phenylpropanoids and essential oil components in basil (Ocimum basilicum L.). Phytochemistry 139:1–7. https://doi.org/10.1016/j.phytochem.2017.03.006
Mierziak J, Kostyn K, Kulma A (2014) Flavonoids as important molecules of plant interactions with the environment. Molecules 19(10):16240–16265
Naik P, Wang JP, Sederoff R et al (2018) Assessing the impact of the 4CL enzyme complex on the robustness of monolignol biosynthesis using metabolic pathway analysis. PLoS One 13(3):e0193896. https://doi.org/10.1371/journal.pone.0193896
Oliveira MB, Andrade RV, Grossi de Sá MF et al (2015) Analysis of genes that are differentially expressed during the Sclerotinia sclerotiorum–Phaseolus vulgaris interaction. Front Microbiol 6:1162. https://doi.org/10.3389/fmicb.2015.01162
Panche AN, Diwan AD, Chandra SR (2016) Flavonoids: an overview. J Nutr Sci 5:e47. https://doi.org/10.1017/jns.2016.41
Peng XQ, Ke AW, Liu JQ et al (2016) Deletion and hormone induction analyses of the 4-coumarate: CoA ligase gene promoter from Pennisetum purpureum in transgenic tobacco plants. Plant Cell Tiss Organ Cult 126:439–448. https://doi.org/10.1007/s11240-016-1012-7
Petersen M, Simmonds MSJ (2003) Rosmarinic acid. Phytochemistry 62:121–125. https://doi.org/10.1016/S0031-9422(02)00513-7
Rani A, Singh K, Sood P et al (2009) p-coumarate:CoA ligase as a key gene in the yield of catechins in tea [Camellia sinensis (L.) O. Kuntze]. Funct Integr Genomics 9:271–275. https://doi.org/10.1007/s10142-008-0098-3
Rao G, Pan X, Xu F et al (2015) Divergent and overlapping function of five 4-Coumarate: coenzyme A ligases from Populus tomentosa. Plant Mol Biol Rep 33:841–854. https://doi.org/10.1007/s11105-014-0803-4
Rastogi S, Kumar R, Chanotiya CS et al (2013) 4-Coumarate:CoA Ligase partitions metabolites for eugenol biosynthesis. Plant Cell Physiol 54(8):1238–1252. https://doi.org/10.1093/pcp/pct073
Saewan N, Jimtaisong A (2013) Photoprotection of natural flavonoids. J Appl Pharm Sci 3(09):129–141. https://doi.org/10.7324/JAPS.2013.3923
Schmelz S, Naismith JH (2009) Adenylate-forming enzymes. Curr Opin Struct Biol 19(6):666–671. https://doi.org/10.1016/j.sbi.2009.09.004
Schmelzer E, Kruger-Lebus S, Hahlbrock K (1989) Temporal and spatial patterns of gene expression around sites of attempted fungal infection in Parsley Leaves. Plant Cell 1:993–1001. https://doi.org/10.1105/tpc.1.10.993
Schmid J, Amrhein N (1995) Molecular organization of the shikimate pathway in higher plants. Phytochemistry 39:737–749. https://doi.org/10.1016/0031-9422(94)00962-S
Schneider K, Hovel K, Witzel K et al (2003) The substrate specificity-determining amino acid code of 4- coumarate: CoA ligase. Proc Natl Acad Sci USA 100:8601–8606. https://doi.org/10.1073/pnas.1430550100
Shigeto J, Ueda Y, Sasaki S et al (2017) Enzymatic activities for lignin monomer intermediates highlight the biosynthetic pathway of syringyl monomers in Robinia pseudoacacia. J Plant Res 130:203–210. https://doi.org/10.1007/s10265-016-0882-4
Shinde BA, Dholakia BB, Hussain K et al (2017) Dynamic metabolic reprogramming of steroidal glycol-alkaloid and phenylpropanoid biosynthesis may impart early blight resistance in wild tomato (Solanum arcanum Peralta). Plant Mol Biol 95(4–5):411–423. https://doi.org/10.1007/s11103-017-0660-2
Shockey JM, Fulda MS, Browse J (2003) Arabidopsis contains a large superfamily of Acyl-Activating enzymes. Phylogenetic and biochemical analysis reveals a new class of Acyl-Coenzyme A synthetases. Plant Physiol 132:1065–1076. https://doi.org/10.1104/pp.103.020552
Silber MV, Meimberg H, Ebel J (2008) Identification of a 4-coumarate: CoA ligase gene family in the moss, Physcomitrella patens. Phytochemistry 69:2449–2456. https://doi.org/10.1016/j.phytochem.2008.06.014
Singh P, Jayaramaiah RH, Agawane SB et al (2016) Potential dual role of eugenol in inhibiting advanced glycation end products in diabetes: proteomic and mechanistic insights. Sci Rep 6:18798. https://doi.org/10.1038/srep18798
Soltani BM, Ehlting J, Hamberger B et al (2006) Multiple cis-regulatory elements regulate distinct and complex patterns of developmental and wound-induced expression of Arabidopsis thaliana 4CL gene family members. Planta 224:1226–1238. https://doi.org/10.1007/s00425-006-0296-y
Stuible HP, Büttner D, Ehlting J et al (2000) Mutational analysis of 4-coumarate: CoA ligase identifies functionally important amino acids and verifies its close relationship to other adenylate-forming enzymes. FEBS Lett 467:117–122
Sun H, Li Y, Feng S et al (2013) Analysis of five rice 4-coumarate: coenzyme A ligase enzyme activity and stress response for potential roles in lignin and flavonoid biosynthesis in rice. Biochem Biophys Res Commun 430:1151–1156. https://doi.org/10.1016/j.bbrc.2012.12.019
Surjadinata BB, Jacobo-Velázquez DA, Cisneros-Zevallos L (2017) UVA, UVB and UVC light enhances the biosynthesis of phenolic antioxidants in fresh-cut carrot through a synergistic effect with wounding. Molecules 22(4):668. https://doi.org/10.3390/molecules22040668
Sutela S, Hahl T, Tiimonen H et al (2014) Phenolic compounds and expression of 4CL genes in silver birch clones and Pt4CL1a lines. Plus One 9(12):e114434. https://doi.org/10.1371/journal.pone.0114434
Szklarczyk D, Franceschini A, Wyder S et al (2015) STRING v10: protein–protein interaction networks, integrated over the tree of life. Nucleic Acids Res 43(Database issue):D447–D452. https://doi.org/10.1093/nar/gku1003
Tatsis EC, O’Connor SE (2016) New developments in engineering plant metabolic pathways. Curr Opin Biotechnol 42:126–132. https://doi.org/10.1016/j.copbio.2016.04.012
The Arabidopsis Genome Initiative (2000) Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408:796–815. https://doi.org/10.1038/35048692
Tohge T, Watanabe M, Hoefgen R et al (2013) Shikimate and phenylalanine biosynthesis in the green lineage. Front Plant Sci 4:62. https://doi.org/10.3389/fpls.2013.00062
Tzin V, Galili G (2010) The biosynthetic pathways for shikimate and aromatic amino acids in Arabidopsis thaliana. Arabidopsis Book Am Soc Plant Biol 8:e0132. https://doi.org/10.1199/tab.0132
Uhlmann A, Ebel J (1993) Molecular cloning and expression of 4-Coumarate-Coenzyme A Ligase, an enzyme involved in the resistance response of soybean (Glycine max L.) against pathogen attack. Plant Physiol 102:1147–1156. https://doi.org/10.1104/pp.102.4.1147
Vogt T (2010) Phenylpropanoid biosynthesis. Mol Plant 3(1):2–20. https://doi.org/10.1093/mp/ssp106
Wang Y, Yi H, Wang M (2011) Structural and kinetic analysis of the unnatural fusion protein 4-coumaroyl-CoA Ligase: stilbene synthase. J Am Chem Soc 51:20684–20687. https://doi.org/10.1021/ja2085993
Wang B, Sun W, Li Q et al (2015) Genome wide analysis phenylpropanoid biosynthesis genes in Salvia miltiorrhiza. Planta 241:711–725. https://doi.org/10.1007/s00425-014-2212-1
Wang CH, You J, Cai YX et al (2016) Characterization and functional analysis of 4-Coumarate-CoA Ligase genes in Mulberry. PLoS One 11(5):e0155814. https://doi.org/10.1371/journal.pone.0155814.g001
Wei XX, Wang WQ (2004) Evolution of 4-coumarate:coenzyme A ligase (4CL) gene and divergence of Larix (Pinaceae). Mol Phylogenet Evol 31(2):542–553. https://doi.org/10.1016/j.ympev.2003.08.015
Winkel-Shirley B (1999) Evidence for enzyme complexes in the phenylpropanoid and flavonoid pathways. Physiol Plant 107:142–149. https://doi.org/10.1034/j.1399-3054.1999.100119.x
Zhang CH, Ma T, Luo WC et al (2015) Identification of 4CL genes in desert Poplars and their changes in expression in response to salt stress. Genes 6:901–917. https://doi.org/10.3390/genes6030901
Zhao Y, Dong W, Wang K et al (2017) Differential sensitivity of fruit pigmentation to ultraviolet light between two peach cultivars. Front Plant Sci 8:1552. https://doi.org/10.3389/fpls.2017.01552
Acknowledgements
We thank Dr. Jayant Khandare, Actorius Innovations and Research Pvt Ltd., Pune, India for critically reading the manuscript and for the English language suggestions. SGL acknowledge the support from Council of Scientific and Industrial Research (CSIR), New Delhi, India. The work was funded by CSIR-NCL-IGIB Joint Research program under XII Five Year Plan (BSC0124).
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Lavhale, S.G., Kalunke, R.M. & Giri, A.P. Structural, functional and evolutionary diversity of 4-coumarate-CoA ligase in plants. Planta 248, 1063–1078 (2018). https://doi.org/10.1007/s00425-018-2965-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00425-018-2965-z