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Phospholipase Dδ is involved in nitric oxide-induced stomatal closure

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Abstract

Nitric oxide (NO) has recently emerged as a second messenger involved in the complex network of signaling events that regulate stomatal closure. Little is known about the signaling events occurring downstream of NO. Previously, we demonstrated the involvement of phospholipase D (PLD) in NO signaling during stomatal closure. PLDδ, one of the 12 Arabidopsis PLDs, is involved in dehydration stress responses. To investigate the role of PLDδ in NO signaling in guard cells, we analyzed guard cells responses using Arabidopsis wild type and two independent pldδ single mutants. In this work, we show that pldδ mutants failed to close the stomata in response to NO. Treatments with phosphatidic acid, the product of PLD activity, induced stomatal closure in pldδ mutants. Abscisic acid (ABA) signaling in guard cells involved H2O2 and NO production, both required for ABA-induced stomatal closure. pldδ guard cells produced similar NO and H2O2 levels as the wild type in response to ABA. However, ABA- or H2O2-induced stomatal closure was impaired in pldδ plants. These data indicate that PLDδ is downstream of NO and H2O2 in ABA-induced stomatal closure.

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Abbreviations

ABA:

Abscisic acid

AU:

Arbitrary unit

DAF-2-DA:

4,5-Diaminofluorescein diacetate

GSNO:

S-nitrosoglutathione

H2DCFDA:

2′,7′,-Dichlorofluorescein diacetate

NO:

Nitric oxide

PA:

Phosphatidic acid

PE:

Phosphatidylethanolamine

PLD:

Phospholipase D

ROS:

Reactive oxygen species

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Acknowledgments

We thank Teun Munnik and Bastiann Bargmann (Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands) for providing us the mutants seeds stocks. This work was financially supported by UNMdP (AML, LL), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) (AMD, AML, CGM, LL), and Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT) (AML, CGM, LL).

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Authors and Affiliations

  1. Instituto de Investigaciones Biológicas, Universidad Nacional de Mar del Plata, CC 1245, 7600, Mar del Plata, Argentina

    Ayelen M. Distéfano, Denise Scuffi, Carlos García-Mata, Lorenzo Lamattina & Ana M. Laxalt

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  1. Ayelen M. Distéfano
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  2. Denise Scuffi
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  3. Carlos García-Mata
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  4. Lorenzo Lamattina
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  5. Ana M. Laxalt
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Correspondence to Ana M. Laxalt.

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Distéfano, A.M., Scuffi, D., García-Mata, C. et al. Phospholipase Dδ is involved in nitric oxide-induced stomatal closure. Planta 236, 1899–1907 (2012). https://doi.org/10.1007/s00425-012-1745-4

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