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Activation of a flavin monooxygenase gene YUCCA7 enhances drought resistance in Arabidopsis

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Abstract

Auxin regulates diverse molecular and physiological events at the cellular and organismal levels during plant growth and development in response to environmental stimuli. It acts either through distinct signaling pathways or in concert with other growth hormones. Its biological functions are adjusted by modulating biosynthesis, conjugate formation, and polar transport and distribution. Several tryptophan-dependent and -independent auxin biosynthetic pathways have been proposed. Recent studies have shown that a few flavin monooxygenase enzymes contribute to the tryptophan-dependent auxin biosynthesis. Here, we show that activation of a flavin monooxygenase gene YUCCA7 (YUC7), which belongs to the tryptophan-dependent auxin biosynthetic pathway, enhances drought resistance. An Arabidopsis activation-tagged mutant yuc7-1D exhibited phenotypic changes similar to those observed in auxin-overproducing mutants, such as tall, slender stems and curled, narrow leaves. Accordingly, endogenous levels of total auxin were elevated in the mutant. The YUC7 gene was induced by drought, primarily in the roots, in an abscisic acid (ABA)-dependent manner. The yuc7-1D mutant was resistant to drought, and drought-responsive genes, such as RESPONSIVE TO DESSICATION 29A (RD29A) and COLD-REGULATED 15A (COR15A), were up-regulated in the mutant. Interestingly, whereas stomatal aperture and production of osmoprotectants were not discernibly altered, lateral root growth was significantly promoted in the yuc7-1D mutant when grown under drought conditions. These observations support that elevation of auxin levels in the roots enhances drought resistance possibly by promoting root growth.

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Abbreviations

ABA:

Abscisic acid

CaMV:

Cauliflower Mosaic Virus

COR:

Cold-regulated

2,4-D:

2,4-Dichlorophenoxyacetic acid

FZY:

FLOOZY

5-MT:

5-Methyl tryptophan

IAA:

Indole-3-acetic acid

qRT-PCR:

Quantitative real-time RT-PCR

RD29A:

RESPONSIVE TO DESSICATION 29A

SEM:

Scanning electron microscopy

TAIL-PCR:

Thermal asymmetric interlaced PCR

WESO1:

WES1

YUC:

YUCCA

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Acknowledgments

This work was supported by the Leaping Research Program (20110016440) provided by the National Research Foundation of Korea, the Next-Generation BioGreen 21 program (Plant Molecular Breeding Center No. PJ008103) provided by the Rural Development Administration, and by grants from the Plant Signaling Network Research Center (20110001099), the National Research Foundation of Korea (20110027355), and the Agricultural R & D Promotion Center (309017-03), Korea Ministry for Food, Agriculture, Forestry and Fisheries.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Chemistry, Seoul National University, Seoul, 151-742, Korea

    Minyoung Lee, Jae-Hoon Jung, Pil Joon Seo & Chung-Mo Park

  2. Brain Korea 21 Graduate Program for RNA Biology, Department of Molecular Biology, Institute of Nanosensor and Biotechnology, Dankook University, Yongin, 448-701, Korea

    Doo-Yeol Han & Woong June Park

  3. Plant Genomics and Breeding Institute, Seoul National University, Seoul, 151-742, Korea

    Chung-Mo Park

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  1. Minyoung Lee
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Correspondence to Chung-Mo Park.

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M. Lee and J. H. Jung contributed equally to the article.

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Lee, M., Jung, JH., Han, DY. et al. Activation of a flavin monooxygenase gene YUCCA7 enhances drought resistance in Arabidopsis . Planta 235, 923–938 (2012). https://doi.org/10.1007/s00425-011-1552-3

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