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Sweetpotato late embryogenesis abundant 14 (IbLEA14) gene influences lignification and increases osmotic- and salt stress-tolerance of transgenic calli

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Abstract

Late embryogenesis abundant 14 (LEA14) cDNA was isolated from an EST library prepared from dehydration-treated fibrous roots of sweetpotato (Ipomoea batatas). Quantitative RT-PCR revealed a variety of different IbLEA14 expression patterns under various abiotic stress conditions. IbLEA14 expression was strongly induced by dehydration, NaCl and abscisic acid treatments in sweetpotato plants. Transgenic sweetpotato non-embryogenic calli harboring IbLEA14 overexpression or RNAi vectors under the control of CaMV 35S promoter were generated. Transgenic calli overexpressing IbLEA14 showed enhanced tolerance to drought and salt stress, whereas RNAi calli exhibited increased stress sensitivity. Under normal culture conditions, lignin contents increased in IbLEA14-overexpressing calli because of the increased expression of a variety of monolignol biosynthesis-related genes. Stress treatments elicited higher expression levels of the gene encoding cinnamyl alcohol dehydrogenase in IbLEA14-overexpressing lines than in control or RNAi lines. These results suggest that IbLEA14 might positively regulate the response to various stresses by enhancing lignification.

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Abbreviations

ABA:

Abscisic acid

CAD:

Cinnamyl alcohol dehydrogenase

CCAOMT:

Caffeoyl coenzyme A O-methyl-transferase

COMT:

Caffeic acid-O-methyl-transferase

C4H:

Cinnamate 4-hydroxylase

DW:

Dry weight

4CL:

4-Coumarate:coenzyme A ligase

LEA:

Late embryogenesis abundant

PAL:

Phenylalanine ammonia-lyase

WC:

Water content

SAMS:

s-Adenosyl-l-methionine synthetase

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Acknowledgments

We are grateful to Dr. Tsuyoshi Nakagawa (Shimane University) for providing the vector PGWB12 and Dr. Sarah M. Assmann (Pennsylvania State University) for pH7GWIW2(I) vector. This work was supported by grants from the Biogreen21 Program (20070301034015), Rural Development Administration, Korea; Korea-China Joint Research Program, National Research Foundation of Korea (NRF); MEST and the KRIBB initiative program.

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Authors and Affiliations

  1. Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Oun-dong 52, Yusong-gu, Daejeon, 305-806, Republic of Korea

    Sung-Chul Park, Yun-Hee Kim, Jae Cheol Jeong, Haeng-Soon Lee & Sang-Soo Kwak

  2. Bioindustrial Process Center Jeonbuk Branch Institute of Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeonbuk, 580-185, Republic of Korea

    Cha Young Kim

  3. Department of Biology, Chungnam National University, Daejeon, 305-764, Republic of Korea

    Sung-Chul Park & Jae-Wook Bang

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  1. Sung-Chul Park
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  2. Yun-Hee Kim
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  4. Cha Young Kim
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Correspondence to Sang-Soo Kwak.

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S.-C. Park and Y.-H. Kim contributed equally to this work.

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Park, SC., Kim, YH., Jeong, J.C. et al. Sweetpotato late embryogenesis abundant 14 (IbLEA14) gene influences lignification and increases osmotic- and salt stress-tolerance of transgenic calli. Planta 233, 621–634 (2011). https://doi.org/10.1007/s00425-010-1326-3

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