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Plant NAC-type transcription factor proteins contain a NARD domain for repression of transcriptional activation

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Abstract

Plant-specific transcription factor NAC proteins play essential roles in many biological processes such as development, senescence, morphogenesis, and stress signal transduction pathways. In the NAC family, some members function as transcription activators while others act as repressors. In the present study we found that though the full-length GmNAC20 from soybean did not have transcriptional activation activity, the carboxy-terminal activation domain of GmNAC20 had high transcriptional activation activity in the yeast assay system. Deletion experiments revealed an active repression domain with 35 amino acids, named NARD (NAC Repression Domain), in the d subdomain of NAC DNA-binding domain. NARD can reduce the transcriptional activation ability of diverse transcription factors when fused to either the amino-terminal or the carboxy-terminal of the transcription factors. NARD-like sequences are also present in other NAC family members and they are functional repression domain when fused to VP16 in plant protoplast assay system. Mutation analysis of conserved amino acid residues in NARD showed that the hydrophobic LVFY motif may partially contribute to the repression function. It is hypothesized that the interactions between the repression domain NARD and the carboxy-terminal activation domain may finally determine the ability of NAC family proteins to regulate downstream gene expressions.

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Abbreviations

ACC:

1-Aminocyclopropane-1-carboxylic acid

ANAC:

Arabidopsis NAC

AREB:

ABA-responsive element binding protein

C/EBP:

CCAAT/enhancer binding protein

DREB/CBF:

Dehydration responsive element/C-repeat binding protein

CBNAC:

Calmodulin-binding NAC protein

CUC:

Cup-shaped cotyledon

ERF:

Ethylene response factor

GRAB:

Geminivirus Rep A-binding

HRT:

Hordeum repressor of transcription

NAC:

NAM, ATAF, and CUC

NAM:

No apical meristem

NAP:

NAC-like, activated by AP3/PI

NARD:

NAC repression domain

NST1:

NAC secondary wall thickening promoting factor1

ONPG:

o-Nitrophenyl β-d-galactopyranoside

PEG:

Polyethylene glycol

RD26:

Responsive to desiccation 26

SNAC:

Stress responsive NAC

X-Gal:

5-Bromo-4-chloro-3-indolyl-beta-d-galactopyranoside

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Acknowledgments

This work was supported by 863 projects (2006AA10A111, 2006AA10Z1F4, 2007AA021402), the National Basic Research Program of China (2006CB100102), the Transgenic Research Projects (2008ZX08009-004), National Key Basic Research Project (2009CB118402) and National Transgenic Research Project (2008ZX08009-003).

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Authors and Affiliations

  1. Plant Gene Research Center, National Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China

    Yu-Jun Hao, Qing-Xin Song, Hao-Wei Chen, Hong-Feng Zou, Wei Wei, Xu-Sheng Kang, Biao Ma, Wan-Ke Zhang, Jin-Song Zhang & Shou-Yi Chen

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  1. Yu-Jun Hao
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  2. Qing-Xin Song
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Correspondence to Wan-Ke Zhang, Jin-Song Zhang or Shou-Yi Chen.

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Hao, YJ., Song, QX., Chen, HW. et al. Plant NAC-type transcription factor proteins contain a NARD domain for repression of transcriptional activation. Planta 232, 1033–1043 (2010). https://doi.org/10.1007/s00425-010-1238-2

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