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Dehydrins expression related to timing of bud burst in Norway spruce

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Abstract

Cold deacclimation and preparation to flushing likely requires rehydration of meristems. Therefore, water stress related genes, such as dehydrins (DHN), might play an important role in providing protection during winter dormancy, deacclimation and bud burst timing processes. Here we report the sequence analysis of several Norway spruce DHN identified in late and early flushing suppressive subtraction hybridization cDNA libraries and in our Norway spruce EST database. We obtained 15 cDNAs, representing eight genes from three distinct types of DHN, and studied differential expression of these genes before and during bud burst in spring, using qRT-PCR. We found the visible reduction in transcript level of most DHN towards the bud burst, supported by a significant down-regulation of the DHN in needles during experimental induction of bud burst applied at three time points during autumn in Norway spruce grafts. For most of the DHN transcripts, their expression levels in late-flushing spruces were significantly higher than in the early flushing ones at the same calendar dates but were remarkably similar at the same bud developmental stage. From our results we may conclude that the difference between the early and the late families is in timing of the molecular processes leading to bud burst due to differences in their response to the increasing temperature in the spring. They are induced much earlier in the early flushing families.

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Abbreviations

EF:

Early flushing family

LF:

Late flushing family

SSH:

Suppression subtractive hybridization

ISSR:

Inter simple sequence repeat

TDF:

Transcript derived fragments

ORF:

Open reading frame

qRT-PCR:

Quantitative reverse transcription-polymerase chain reaction

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Acknowledgments

We wish to thank Veronika Yakovleva for her help and support during sample collection. We also thank Heikki Hänninen and Risto Häkkinen (both at Finnish Forest Research Institute) for their contribution in planning of the graft production in Finland. The project was supported by the Research Council of Norway (Grants No 143276/110, No 155041/140 and No 155873/720) and the Nordic Forest Research Co-operation Committee (SNS 99 and CAR 4 2005; GENECAR).

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Authors and Affiliations

  1. The Norwegian Forest and Landscape Institute, Høgskoleveien 8, 1432, Ås, Norway

    Igor A. Yakovlev, Daniel K. A. Asante, Carl Gunnar Fossdal & Øystein Johnsen

  2. Finnish Forest Research Institute (METLA), Punkaharju Research Unit, Finlandiantie 18, 58450, Punkaharju, Finland

    Jouni Partanen

  3. Department of Biology, University of Tromsø, 9037, Tromso, Norway

    Daniel K. A. Asante & Olavi Junttila

Authors
  1. Igor A. Yakovlev
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  2. Daniel K. A. Asante
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  3. Carl Gunnar Fossdal
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  5. Olavi Junttila
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  6. Øystein Johnsen
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Correspondence to Igor A. Yakovlev.

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Yakovlev, I.A., Asante, D.K.A., Fossdal, C.G. et al. Dehydrins expression related to timing of bud burst in Norway spruce. Planta 228, 459–472 (2008). https://doi.org/10.1007/s00425-008-0750-0

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