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Characterization of PsMPK2, the first C1 subgroup MAP kinase from pea (Pisum sativum L.)

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Abstract

Mitogen-activated protein kinase (MAPK) cascades play a key role in plant growth and development as well as in biotic and abiotic stress responses. They are classified according to their sequence homology into four major groups (A–D). A large amount of information about MAPKs in groups A and B is available but few data of the C group have been reported. In this study, a C1 subgroup MAP kinase cDNA, PsMPK2, was isolated from Pisum sativum. PsMPK2 is expressed in vegetative (root and leaf) and reproductive (stamen, pistil and fruit) organs. Expression of PsMPK2 in Arabidopsis thaliana shows that mechanical injury and other stress signals as abscisic acid, jasmonic acid and hydrogen peroxide increase its kinase activity, extending previous results indicating that C1 subgroup MAPKs may be involved in the response to stress.

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Abbreviations

ABA:

Abscisic acid

JA:

Jasmonic acid

MAPK:

Mitogen-activated protein kinase

MBP:

Myelin basic protein

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Acknowledgments

Help from MA Argomániz for the preparation of GST-PsMPK2, Dr. E. Grau with automatic DNA sequencing, Dr. M. D Gómez with the stereoscopic microscope and R. Martínez-Pardo and A. Villar in the greenhouse is acknowledged (all of them from the Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas y Universidad Politécnica de Valencia, Spain, where the initial part of this work was done). We thank Dr. MA Blázquez (Instituto de Biología Molecular y Celular de Plantas, Valencia, Spain) for critical reading of the manuscript. This work was supported by grants GVDOC00-24-04 and CTIDIB/2002/67 from Generalitat Valenciana, BIO2002-04083-C03-01 from Ministerio de Ciencia y Tecnología and BFU2005-00071/BMC and BFU2006-01149/BMC from Ministerio de Educación y Ciencia, Spain. D. Ortiz-Masia was supported by a “V Segles” pre-doctoral fellowship from Universidad de Valencia and MJ Marcote is a Ramón y Cajal researcher.

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Authors and Affiliations

  1. Facultad de Farmacia, Universidad de Valencia, Avda. Vicente Andrés Estellés s/n, 46100, Burjassot, Spain

    Dolores Ortiz-Masia, Fernando Aniento & Maria J. Marcote

  2. Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas y Universidad Politécnica de Valencia, Avda. de los Naranjos s/n, 46022, Valencia, Spain

    Miguel A. Perez-Amador, Pablo Carbonell & Juan Carbonell

Authors
  1. Dolores Ortiz-Masia
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  2. Miguel A. Perez-Amador
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  3. Pablo Carbonell
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  4. Fernando Aniento
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  5. Juan Carbonell
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  6. Maria J. Marcote
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Correspondence to Maria J. Marcote.

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Ortiz-Masia, D., Perez-Amador, M.A., Carbonell, P. et al. Characterization of PsMPK2, the first C1 subgroup MAP kinase from pea (Pisum sativum L.). Planta 227, 1333–1342 (2008). https://doi.org/10.1007/s00425-008-0705-5

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