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Characterization of plant XRCC1 and its interaction with proliferating cell nuclear antigen

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Abstract

In plants, there are no DNA polymerase β (Pol β) and DNA ligase III (Lig3) genes. Thus, the plant short-patch base excision repair (short-patch BER) pathway must differ considerably from that in mammals. We characterized the rice (Oryza Sativa L. cv. Nipponbare) homologue of the mammalian X-ray repair cross complementing 1 (XRCC1), a well-known BER protein. The plant XRCC1 lacks the N-terminal domain (NTD) which is required for Pol β binding and is essential for mammalian cell survival. The recombinant rice XRCC1 (OsXRCC1) protein binds single-stranded DNA (ssDNA) as well as double-stranded DNA (dsDNA) and also interacts with rice proliferating cell nuclear antigen (OsPCNA) in a pull-down assay. Through immunoprecipitation, we demonstrated that OsXRCC1 forms a complex with PCNA in vivo. OsXRCC1 mRNA was expressed in all rice organs and was induced by application of bleomycin, but not of MMS, H2O2 or UV-B. Bleomycin also increased the fraction of OsXRCC1 associated with chromatin. These results suggest that OsXRCC1 contributes to DNA repair pathways that differ from the mammalian BER system.

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Abbreviations

AP sites:

Apurinic/apyrimidinic sites

GFP:

Green fluorescent protein

GST:

Glutathione S-transferase

LpBER:

Long-patch base excision repair

MMS:

Methylmethanesulfonate

SAM:

Shoot apical meristem

SpBER:

Short-patch base excision repair

XRCC1:

X-ray repair cross complementing 1

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Acknowledgments

We express our appreciation to Dr. Y. Niwa (Laboratory of Plant Cell Technology, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka) for providing the CaMV35S-sGFP(S65T)-nos3′ SK vector. Y. U. was supported by a Research Fellowships of the Japan Society for the Promotion of Science.

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  1. Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan

    Yukinobu Uchiyama, Yuko Suzuki & Kengo Sakaguchi

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  1. Yukinobu Uchiyama
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  2. Yuko Suzuki
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  3. Kengo Sakaguchi
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Correspondence to Kengo Sakaguchi.

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Uchiyama, Y., Suzuki, Y. & Sakaguchi, K. Characterization of plant XRCC1 and its interaction with proliferating cell nuclear antigen. Planta 227, 1233–1241 (2008). https://doi.org/10.1007/s00425-008-0695-3

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