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Photosynthetic H2 metabolism in Chlamydomonas reinhardtii (unicellular green algae)

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Abstract

Unicellular green algae have the ability to operate in two distinctly different environments (aerobic and anaerobic), and to photosynthetically generate molecular hydrogen (H2). A recently developed metabolic protocol in the green alga Chlamydomonas reinhardtii permitted separation of photosynthetic O2-evolution and carbon accumulation from anaerobic consumption of cellular metabolites and concomitant photosynthetic H2-evolution. The H2 evolution process was induced upon sulfate nutrient deprivation of the cells, which reversibly inhibits photosystem-II and O2-evolution in their chloroplast. In the absence of O2, and in order to generate ATP, green algae resorted to anaerobic photosynthetic metabolism, evolved H2 in the light and consumed endogenous substrate. This study summarizes recent advances on green algal hydrogen metabolism and discusses avenues of research for the further development of this method. Included is the mechanism of a substantial tenfold starch accumulation in the cells, observed promptly upon S-deprivation, and the regulated starch and protein catabolism during the subsequent H2-evolution. Also discussed is the function of a chloroplast envelope-localized sulfate permease, and the photosynthesis–respiration relationship in green algae as potential tools by which to stabilize and enhance H2 metabolism. In addition to potential practical applications of H2, approaches discussed in this work are beginning to address the biochemistry of anaerobic H2 photoproduction, its genes, proteins, regulation, and communication with other metabolic pathways in microalgae. Photosynthetic H2 production by green algae may hold the promise of generating a renewable fuel from nature’s most plentiful resources, sunlight and water. The process potentially concerns global warming and the question of energy supply and demand.

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Abbreviations

Chl:

Chlorophyll

DCMU:

3-(3′,4′-Dichlorophenyl)-1,1-dimethyl urea

LHC:

Light-harvesting complex

PS:

Photosystem

PAR:

Photosynthetically active radiation

Rubisco:

Ribulose-1,5-bisphosphate carboxylase/oxygenase

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Acknowledgments

Thanks are due to Dr. Hsu-Ching Chen for help with the measurement of H2-production in anti-sulP strains of C. reinhardtii.

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  1. Department of Plant and Microbial Biology, University of California, 111 Koshland Hall, Berkeley, CA, 94720-3102, USA

    Anastasios Melis

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Correspondence to Anastasios Melis.

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Melis, A. Photosynthetic H2 metabolism in Chlamydomonas reinhardtii (unicellular green algae). Planta 226, 1075–1086 (2007). https://doi.org/10.1007/s00425-007-0609-9

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