Abstract
PSI-E is part of the stromal side of photosystem I (PSI). In Arabidopsis thaliana, the two nuclear genes PsaE1 and PsaE2 code for PSI-E, and transcripts of PsaE1 are markedly more abundant than PsaE2 transcripts. Stable null alleles of the two PsaE genes, psae1-3 and psae2-1, were identified and characterised. The psae2-1 mutant exhibited wild-type like PSI-E abundance and photosynthetic performance, whereas in the psae1-3 mutant PSI-E accumulation was decreased by 85%, together with an impaired thylakoid electron flow and plant growth rate. The psae1-3 psae2-1 double mutant totally lacked PSI-E but was still able to grow photoautotrophically, implying that PSI-E is not essential for PSI accumulation and thylakoid electron flow.
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Abbreviations
- LHCI (II):
-
Light-harvesting complex I (II)
- OEC:
-
Oxygen-evolving complex
- PAGE:
-
Polyacrylamide gel electrophoresis
- PSI (II):
-
Photosystem I (II)
- WT:
-
Wild type
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Acknowledgments
The authors thank the Deutsche Forschungsgemeinschaft (III GK—GRK 306/1; DL 1265/9; SFB-TR1 TP-B8) and the European Community’s Human Potential Programme (contract no. HPRN-CT-2002-00248) for financial support.
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A. Ihnatowicz, P. Pesaresi both authors contributed equally to this work.
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Ihnatowicz, A., Pesaresi, P. & Leister, D. The E subunit of photosystem I is not essential for linear electron flow and photoautotrophic growth in Arabidopsis thaliana . Planta 226, 889–895 (2007). https://doi.org/10.1007/s00425-007-0534-y
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DOI: https://doi.org/10.1007/s00425-007-0534-y