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cyclo-Oxylipin-galactolipids in plants: occurrence and dynamics

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Abstract

cyclo-Oxylipin-galactolipids (cGL) are mono- or digalactosyldiglycerides carrying a cyclo-oxylipin in the sn1- and/or sn2-position or esterified to the galactose moiety. These compounds were recently identified in Arabidopsis thaliana. We provide evidence that cGL are mainly, if not exclusively, part of the thylakoid and can be hydrolysed by lipolytic activities associated with photosynthesis-related protein complexes in vitro. Using HPLC/ESI–mass spectrometry, cGL are shown to be restricted in occurrence to the genus Arabidopsis, they do not occur in other plants tested. A. thaliana cGL are rapidly and transiently formed upon wounding with characteristic changes in composition of the cGL-fraction. While the biological role of cGL is not understood, the genus Arabidopsis may present a model-case of chemical evolution of a novel class of regulatory molecules.

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Abbreviations

DGDG:

Digalactosyldiacylglycerol

cGL:

cyclo-Oxylipin-galactolipids

JA:

Jasmonic acid

LHCP:

Light harvesting complex protein

OPDA:

12-Oxophytodienoic acid

MGDG:

Monogalactosyldiacylglycerol

PG:

Phosphatidylglycerol

PSI (II):

Photosystem I (II)

TPT:

Triose phosphate translocator

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Acknowledgments

We thank Jun-Prof. Danja Schünemann (Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr-Universität Bochum, Germany) for providing the LHCP and cpSRP43 antibodies as well as the secondary antibody from chicken. We also like to thank Prof. Ulf-Ingo Flügge (Lehrstuhl für Botanik II, Universität zu Köln, Germany) for the kind provision of the TPT antibody. Furthermore we thank Dr. Markus Piotrowski (Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität Bochum, Germany) for the mass spectrometrical analyses of the thylakoid protein complexes. This work was funded by a grant of the Deutsche Forschungsgemeinschaft (SFB480, TP A8) to EWW.

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  1. Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität Bochum, Universitätsstraße 150, 44801, Bochum, Germany

    Christine Böttcher & Elmar W. Weiler

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  1. Christine Böttcher
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  2. Elmar W. Weiler
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Correspondence to Christine Böttcher.

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Böttcher, C., Weiler, E.W. cyclo-Oxylipin-galactolipids in plants: occurrence and dynamics. Planta 226, 629–637 (2007). https://doi.org/10.1007/s00425-007-0511-5

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