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Glutathione synthesis is regulated by nitric oxide in Medicago truncatula roots

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Abstract

Glutathione (GSH) is one of the main antioxidants in plants. Legumes have the specificity to produce a GSH homolog, homoglutathione (hGSH). We have investigated the regulation of GSH and hGSH synthesis by nitric oxide (NO) in Medicago truncatula roots. Analysis of the expression level of gamma-glutamylcysteine synthetase (γ-ECS), glutathione synthetase (GSHS) and homoglutathione synthetase (hGSHS) after treatment with sodium nitroprusside (SNP) and nitrosoglutathione (GSNO), two NO-donors, showed that γ-ecs and gshs genes are up regulated by NO treatment whereas hgshs expression is not. Differential accumulation of GSH was correlated to gene expression in SNP-treated roots. Our results provide the first evidence that GSH synthesis pathway is regulated by NO in plants and that there is a differential regulation between gshs and hgshs in M. truncatula.

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Fig. 1
Fig. 2

Abbreviations

γ-EC:

Gamma-glutamylcysteine

γ-ECS:

Gamma-glutamylcysteine synthetase

FeCN:

Ferricyanide

GSH:

Glutathione

GSHS:

Glutathione synthetase

GSNO:

Nitrosoglutathione

hGSH:

Homoglutathione

hGSHS:

Homoglutathione synthetase

JA:

Jasmonic acid

NO:

Nitric oxide

SNP:

Sodium nitroprusside

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Acknowledgments

Chiara Pucciariello is the recipient of a Marie Curie fellowship.

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Authors and Affiliations

  1. Interactions Plantes-Microorganismes et Santé Végétale, UMR INRA 1064/Université de Nice-Sophia Antipolis/CNRS 6192, 400, route des Chappes, BP 167, 06903, Sophia-Antipolis, France

    Gilles Innocenti, Chiara Pucciariello, Marie Le Gleuher, Julie Hopkins, Alain Puppo & Pierre Frendo

  2. Laboratoire de Biologie Cellulaire et Moléculaire des Plantes, FRE 2846 UPMC/CNRS, Université Pierre et Marie Curie, Paris 6, 4, rue Galilée, 94200, Ivry-sur-Seine, France

    Emmanuel Baudouin

  3. Dipartimento scientifico e tecnologico, Università degli studi di Verona, Strada Le Grazie 15, 37134, Verona, Italy

    Matteo de Stefano & Massimo Delledonne

Authors
  1. Gilles Innocenti
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  2. Chiara Pucciariello
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  3. Marie Le Gleuher
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  4. Julie Hopkins
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  5. Matteo de Stefano
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  6. Massimo Delledonne
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  7. Alain Puppo
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  8. Emmanuel Baudouin
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  9. Pierre Frendo
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Corresponding author

Correspondence to Alain Puppo.

Additional information

G. Innocenti and C. Pucciariello have contributed equally to the work.

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Innocenti, G., Pucciariello, C., Le Gleuher, M. et al. Glutathione synthesis is regulated by nitric oxide in Medicago truncatula roots. Planta 225, 1597–1602 (2007). https://doi.org/10.1007/s00425-006-0461-3

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  • DOI: https://doi.org/10.1007/s00425-006-0461-3

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