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Hydrogen peroxide production is an early event during bicarbonate induced stomatal closure in abaxial epidermis of Arabidopsis

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Abstract

The presence of 2 mM bicarbonate in the incubation medium induced stomatal closure in abaxial epidermis of Arabidopsis. Exposure to 2 mM bicarbonate elevated the levels of H2O2 in guard cells within 5 min, as indicated by the fluorescent probe, dichlorofluorescein diacetate (H2DCF-DA). Bicarbonate-induced stomatal closure as well as H2O2 production were restricted by exogenous catalase or diphenylene iodonium (DPI, an inhibitor of NAD(P)H oxidase). The reduced sensitivity of stomata to bicarbonate and H2O2 production in homozygous atrbohD/F double mutant of Arabidopsis confirmed that NADP(H) oxidase is involved during bicarbonate induced ROS production in guard cells. The production of H2O2 was quicker and greater with ABA than that with bicarbonate. Such pattern of H2O2 production may be one of the reasons for ABA being more effective than bicarbonate, in promoting stomatal closure. Our results demonstrate that H2O2 is an essential secondary messenger during bicarbonate induced stomatal closure in Arabidopsis.

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Abbreviations

ABA:

Abscisic acid

DPI:

Diphenylene iodonium

H2DCF-DA:

Dichlorofluorescein diacetate

MJ:

Methyl jasmonate

PI3P:

Phosphatidylinositol 3-phosphate

ROS:

Reactive oxygen species

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Acknowledgments

This work was supported by grants from Indo-French Centre for the promotion of Advanced Research (to A.S.R and A.V, No. 2203-1) and Council of Scientific and Industrial Research (No. 38(0949)/99/EMR-II) both from New Delhi; and a senior research fellowship (to V.A.K) from Institute of Life Science Grant to University of Hyderabad. We thank Professor Julian Schroeder for providing the atrbohD/F double mutant seeds of Arabidopsis. We thank C. S. Murthy, Sr. Scientific Officer, Central Instrumentation Laboratory for his constant help in using confocal microscope.

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Authors and Affiliations

  1. Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, 500 046, India

    V. A. Kolla & A. S. Raghavendra

  2. CEA/Cadarache-DSV-DEVM, Laboratoire des Echanges Membranaires et Signalisation, UMR 6191 CNRS-CEA, Universitée de la Meéditerranée, 13108 St Paul lez Durance, Cedex, France

    A. Vavasseur

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  1. V. A. Kolla
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  3. A. S. Raghavendra
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Correspondence to A. S. Raghavendra.

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Kolla, V.A., Vavasseur, A. & Raghavendra, A.S. Hydrogen peroxide production is an early event during bicarbonate induced stomatal closure in abaxial epidermis of Arabidopsis . Planta 225, 1421–1429 (2007). https://doi.org/10.1007/s00425-006-0450-6

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