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Singlet oxygen affects the activity of the thylakoid ATP synthase and has a strong impact on its γ subunit

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Abstract

Singlet oxygen is reported to have the most potent damaging effect upon the photosynthetic machinery. Usually this reactive oxygen molecule acts in concert with other ROS types under stressful conditions. To understand the specific role of singlet oxygen we took advantage of the conditional flu mutant of Arabidopsis thaliana. In flu, the negative feedback loop is abolished, which blocks chlorophyll biosynthesis in the dark. Therefore high amounts of free protochlorophyllide accumulate during darkness. If flu gets subsequently illuminated, free protochlorophyllide acts as a photosensitiser leading almost exclusively to high amounts of 1O2. Analysing the thylakoid protein pattern by using 2D PAGE and subsequent MALDI-TOF analysis, we could show, in addition to previous described effects on photosystem II, that singlet oxygen has a massive impact on the thylakoid ATP synthase, especially on its γ subunit. Additionally, it could be shown that the activity of the ATP synthase is reduced upon singlet oxygen exposure and that the rate of non-photochemical quenching is affected in flu mutants exposed to 1O2.

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Abbreviations

2D PAGE:

Two-dimensional polyacrylamide gel electrophoresis

ATP:

Adenosine triphosphate

LUC:

Firefly luciferase

MALDI-TOF:

Matrix-assisted laser desorption/ionisation time-of-flight

NPQ:

Non-photochemical quenching

1O2 :

Singlet oxygen

ROS:

Reactive oxygen species

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Acknowledgments

We would like to thank Klaus Apel (ETH Zurich, Switzerland) for providing the flu mutant, Jon Hughes (Justus-Liebig University, Giessen, Germany) for critical reading of the manuscript and many helpful discussions. We are gratefully indebted to Melanie Bingel and André Imboden for technical assistance. This work was supported by the Justus-Liebig University, Giessen and in parts by the Swiss National Science Foundation (K. Apel).

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Authors and Affiliations

  1. Department of Plant Physiology, Justus-Liebig Universität, Senckenbergstr. 3, Giessen, 35390, Germany

    Hanno Mahler & Christoph Forreiter

  2. Department of Plant Sciences, ETH Zurich, Universitätstr. 2, Zurich, 8092, Switzerland

    Hanno Mahler, Dominika Przybyla & Frank Landgraf

  3. Department of Molecular Plant Physiology, Friedrich-Alexander Universität, Staudtstr. 5, Erlangen, 91058, Germany

    Petra Wuennenberg

  4. Department of Biochemistry, Justus-Liebig Universität, Friedrichstrasse 24, Giessen, 35392, Germany

    Monica Linder

  5. Department of Plant Nutrition, Justus-Liebig Universität, Heinrich-Buff-Ring 26-32, Giessen, 35392, Germany

    Christian Zoerb

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  1. Hanno Mahler
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  2. Petra Wuennenberg
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  3. Monica Linder
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  4. Dominika Przybyla
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  5. Christian Zoerb
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  6. Frank Landgraf
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  7. Christoph Forreiter
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Correspondence to Christoph Forreiter.

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Mahler, H., Wuennenberg, P., Linder, M. et al. Singlet oxygen affects the activity of the thylakoid ATP synthase and has a strong impact on its γ subunit. Planta 225, 1073–1083 (2007). https://doi.org/10.1007/s00425-006-0416-8

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  • DOI: https://doi.org/10.1007/s00425-006-0416-8

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