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Major differences observed in transcript profiles of blueberry during cold acclimation under field and cold room conditions

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Abstract

Our laboratory has been working toward increasing our understanding of the genetic control of cold hardiness in blueberry (Vaccinium section Cyanococcus) to ultimately use this information to develop more cold hardy cultivars for the industry. Here, we report using cDNA microarrays to monitor changes in gene expression at multiple times during cold acclimation under field and cold room conditions. Microarrays contained over 2,500 cDNA inserts, approximately half of which had been picked and single-pass sequenced from each of two cDNA libraries that were constructed from cold acclimated floral buds and non-acclimated floral buds of the fairly cold hardy cv. Bluecrop (Vaccinium corymbosum L.). Two biological samples were examined at each time point. Microarray data were analyzed statistically using t tests, ANOVA, clustering algorithms, and online analytical processing (OLAP). Interestingly, more transcripts were found to be upregulated under cold room conditions than under field conditions. Many of the genes induced only under cold room conditions could be divided into three major types: (1) genes associated with stress tolerance; (2) those that encode glycolytic and TCA cycle enzymes, and (3) those associated with protein synthesis machinery. A few of the genes induced only under field conditions appear to be related to light stress. Possible explanations for these differences are discussed in physiological context. Although many similarities exist in how plants respond during cold acclimation in the cold room and in the field environment, there are major differences suggesting caution should be taken in interpreting results based only on artificial, cold room conditions.

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Abbreviations

CA:

Cold acclimated

EST:

Expressed sequence tag

NA:

Non-acclimated

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Authors and Affiliations

  1. Fruit Laboratory, USDA/ARS, Henry A. Wallace Beltsville Agricultural Research Center, Bldg. 010A, BARC-West, 10300 Baltimore Ave., Beltsville, MD, 20705, USA

    Anik L. Dhanaraj & Lisa J. Rowland

  2. Department of Computer and Information Sciences, Towson University, Towson, MD, 21252, USA

    Nadim W. Alkharouf

  3. Soybean Genomics and Improvement Laboratory, USDA/ARS, Henry A. Wallace Beltsville Agricultural Research Center, Beltsville, MD, 20705, USA

    Hunter S. Beard, Imed B. Chouikha & Benjamin F. Matthews

  4. Department of Horticulture, Iowa State University, Ames, IA, 50011, USA

    Hui Wei & Rajeev Arora

Authors
  1. Anik L. Dhanaraj
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  2. Nadim W. Alkharouf
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  3. Hunter S. Beard
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  4. Imed B. Chouikha
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  5. Benjamin F. Matthews
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  6. Hui Wei
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  7. Rajeev Arora
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  8. Lisa J. Rowland
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Correspondence to Lisa J. Rowland.

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Dhanaraj, A.L., Alkharouf, N.W., Beard, H.S. et al. Major differences observed in transcript profiles of blueberry during cold acclimation under field and cold room conditions. Planta 225, 735–751 (2007). https://doi.org/10.1007/s00425-006-0382-1

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  • DOI: https://doi.org/10.1007/s00425-006-0382-1

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