Abstract
Inorganic phosphate (Pi) uptake across the vacuolar membrane of intact vacuoles isolated from Catharanthus roseus suspension-cultured cells was measured. Under low Pi status, Pi uptake into the vacuole was strongly activated compared to high Pi status. Since Pi uptake across the vacuolar membrane is correlated with H+ pumping, we examined the dependency of H+ pumping on plant Pi status. Both H+ pumping and the activities of the vacuolar H+-pumps, the V-type H+-ATPase and the H+-PPase were enhanced under low Pi status. Despite this increase in H+ pumping, Western blot analysis showed no distinct increase in the amount of proton pump proteins. Possible mechanisms for the activation of Pi uptake into the vacuole under low Pi status are discussed.
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Abbreviations
- Pi:
-
Inorganic phosphate
- MS:
-
Murashige–Skoog medium
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Acknowledgments
We greatly appreciate Dr Rob Reid (University of Adelaide, Adelaide, Australia) for his kind discussion and correction of this manuscript. This work was supported by a Grant-in-Aid for Scientific Research on Priority Areas (10219202 and 16085204) and a Grant-in-Aid for Exploratory Research (14654165) by the Japanese Ministry of Education, Culture, Sports, Science and Technology, and Japan Society for the Promotion of Science, and CREST of JST (Japan Science and Technology Corporation). This collaborative work was also supported by the Swiss National Foundation.
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Miwa Ohnishi and Tetsuro Mimura contributed equally to this work.
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Ohnishi, M., Mimura, T., Tsujimura, T. et al. Inorganic phosphate uptake in intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus (L.) G. Don under varying Pi status. Planta 225, 711–718 (2007). https://doi.org/10.1007/s00425-006-0379-9
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DOI: https://doi.org/10.1007/s00425-006-0379-9